微生物转化苯乙酮酸合成R扁桃酸-福州大学学报.PDFVIP

34 3 ( ) V l. 34 N . 3 2006 6 J urnal f Fuzh u University( Natural Science) Jun. 2006 : 1000- 2243( 2006) 03- 0435- 04 R() 1 1 2 , , ( 1. , 362021; 2. , 350002) : FD11b R(). FD11b 6 ( 14 d) , , R() ( e. e. ) 96. 5% . 30 L Fed- Batch , ( , ) 12. 5 gL, 20~ 30mm lL , 49 h, 125 mm l L, 96. 1% , e. e. 98. 5% . : ; R() ; ; : O621. 34 : A Microbial transformation of phenylglyoxilic acid to R()mandelic acid by Saccharomyces cerevisiae FD11b 1 1 2 HUANG Ya- yan , XIAO Mei- tian , GUO Yang- ha (1. Department f Chemical and Pharmaceutical Engineering, Huaqia University, Quanzh u, Fujian 362021, China; 2. Institute f Pharmaceutical Bi techn l gy and Engineering, Fuzh u University, Fuzh u, Fujian 350002, China) Abstract: The reusable capability f S accharomyces cerevis iae FD11b was determined , which was used in the asymmetric reducti n f phenylgly xilic acid t R()mandelic acid. In the peri d f six c ntinu us batches( 14 days) , FD11b kept a high catalytic reducti n activity and the e. e. value f R() mandelic acid was higher than 96. 5% in each ne. When the bi mass c ncentrati n was 12. 5gL ( cell dry weighreactive v lume) and the c ncentrati n f substrate phenylgly xilic acid was c ntr lled between 20 and 30mm lL, the c ncentrati n f pr duct mandelic acid f 125 mm lL c uld be achieved in 30 L ferment r after the reducti n f 49 h, and the yield and enanti meric excess f mandelic acid were 96. 1% and 98. 3% , respectively. Keywords: micr bial transf rmati n;