无花果多糖的纯化及其抗氧化活性研究.docxVIP

无花果多糖的纯化及其抗氧化活性研究李文婕1，杨小明1，*，张赫男1，李世超1，方洋洋2（1.江苏大学食品与生物工程学院，江苏镇江212013；2.江苏大学化学化工学院，江苏镇江212013）摘 要：目的：探讨无花果多糖（Ficus caricapolysaccharides，FCPS）的分离纯化及其抗氧化活性。方法：采用水提醇沉法提取，Sevage法除蛋白、膜过滤后冻干得到无花果多糖，再经DEAE-52纤维素柱纯化；以UV等方法考察多糖的理化性质，高效凝胶色谱测定多糖的纯度和相对分子质量分布；并研究其抗氧化活性。结果：经水提醇沉、膜过滤后得到的FCPS为混合酸性多糖，不含蛋白质、核酸，分子量范围在5.92×105~2.0×106u之间；DEAE-52柱分级得到FCPS的三个馏分：FCPS1、FCPS2和FCPS3，高效凝胶色谱分析显示FCPS2为一对称峰，分子量约为2.61×106u。抗氧化活性实验显示清除超氧负离子能力IC50FCPS2为430μg/mL，FCPS为620μg/mL；清除羟基自由基IC50FCPS2为1000μg/mL，FCPS为4780μg/mL。在浓度为1000μg/mL时，FCPS2和FCPS的还原能力分别为0.055和0.133。结论：FCPS经纯化后得到的组分FCPS2较FCPS表现出更好的清除超氧负离子和清除羟基自由基的能力，但还原能力有所降低。关键词：无花果多糖，分离纯化，抗氧化Study on purification and antioxidation of polysaccharidesfromFicuscaricaLIWen-jie1，YANGXiao-ming1，*，ZHANGHe-nan1，LIShi-chao1，FANGYang-yang2（1.SchoolofFoodandBiologicalEngineering，JiangsuUniversity，Zhenjiang212013，China；2.SchoolofChemistryandChemicalEngineering，JiangsuUniversity，Zhenjiang212013，China）Abstract：Objective：TopurifythepolysaccharidesfromFicuscaricaL.fruitandinvestigateitsantioxidantactivity.Methods：Ficuscaricapolysaccharides（FCPS）wasobtainedbyhotwaterextractingandethanolprecipitation，removingtheproteinbySevagemethod.ThenFCPSwaspurifiedbyusingcelluloseDEAE-52.PhysicochemicalcharacteristicofpolysaccharideswasresearchedbyUVandsomeothermethods.HPGPCwasusedtostudythepurityanddistributionrangeofrelativemolecularmassofpolysaccharides.Andtheantioxidantactivityofthepolysaccharideswastested.Results：FCPSwasakindofmixedacidicpolysaccharideswhichhadnoprotein and nucleinic acid. The relative molecular mass was between 5.92×105 and 2.0×106u. The three purifiedcomponentsFCPS1，FCPS2andFCPS3wereobtainedbyDEAE-52cellulosecolumnChromatography.AfterHPGPCanalyses，FCPS2showedasymmetricalpeakanditsmolecularmasswas2.61×106u.Asaboutantioxidation，scavengingsuperoxideanionradical（FCPS2：IC50=430μg/mL，FCPS：IC50＝620μg/mL）andscavenginghydroxylradical（FCPS2：IC50=1000μg/mL，FCPS：IC50＝4780μg/mL）werealsonotedlyimproved，whereas，the reducingpowerwas0.055and0.133respectivelywhentheirconcen