基于渗透压平衡和元素供应的隐甲藻分批式高密度发酵研究.pdfVIP

基于渗透压平衡和元素供应的隐甲藻分批式高密度发 酵研究 1 2 1 1 1,2* 任路静 ，肖爱华 ，练敏 ，金立晶 ，黄和 1 （南京工业大学制药与生命科学学院，江苏南京，210009 ；） 2 （江苏省工业生物技术创新中心，江苏南京，210009 ） 摘要：隐甲藻为一种海洋微生物，是常用的DHA 生产菌。本文以实验室保藏的隐甲藻为研究对象，在高 初糖浓度下，考察隐甲藻对培养环境渗透压条件的耐受性并确定最适渗透压条件为 1715.46 mOsm·L-1， 以此渗透压为基础，从碳、氮、磷、硫四种元素供应角度优化隐甲藻生长环境。结果表明，在碳元素64g/L， 氮元素2.58g/L ，硫元素2.668g/L ，磷元素0.912g/L 条件下，细胞干重达 56.5g/L,，脂肪酸占细胞干重在 31%左右，DHA 占总脂肪酸含量35.34%，DHA 含量达6.189g/L 。 关键词：渗透压 元素供应 隐甲藻 高密度发酵 中图分类号：TQ92 文献标识码： 文章编号： High cell density fermentation of C.cohnii based on osmotic pressure balance and element supply 1 2 1 1 1,2 REN Lujing ，XIAO Aihua ，LIAN Min ，JIN Lijing ，HUANG He 1 ( College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing, 210009, Jiangsu, China) (2 Jiangsu Provincial Innovation Center for Industrial Biotechnology, Nanjing, 210009, Jiangs, China) Abstract: The heterotrophic microalga Crypthecodinium cohnii is a marine microorganism, which is considered to be one of highly promising source for DHA production. In this paper, at the high initial glucose concentration, we investigate the toleration of C. cohnii to osmotic pressure and determine the optimal osmotic pressure for cell growth and lipid accumulation is -1 1715.46 mOsm·L . Based on the optimal osmotic pressure, we also optimized the element supply, and the optimal results showed that cell dry weight of 56.5g/L and percentage of total fatty acids of cell dry weight of 31%, DHA percentage of fatty acid of 35.34% were achieved whe