江西铅山红芽芋胚性愈伤组织的包埋干燥法超低温保存.docx

江西铅山红芽芋胚性愈伤组织的包埋干燥法超低温保存*洪森荣，尹明华＊＊，王艾平( 上饶师范学院生命科学学院，江西 上饶 334001)摘要: 对江西铅山红芽芋 ( Colocasia esculenta var． cormosus cv． Hongyayu) 胚性愈伤组织包埋干燥法超低温保存进行了初步的研究。结果表明: 江西铅山红芽芋胚性愈伤组织包埋干燥法超低温保存较佳的条件为:0. 75 mol·L－1 蔗糖预培养 3 d; 脱水方式为空气干燥 7 h 或硅胶干燥 11 h; 化冻温度为 37 ℃ ( 2 min) ; 冻后 培养条件为暗培养 7 d 再转到光周期中培养。此方法超低温保存后的平均成活率约为 45%。超低温保存时 间以及是否去除包裹的褐藻酸钙对其成活率无显著性影响。形态学和细胞学检测表明红芽芋胚性愈伤组织 冻后再生苗与母本材料相比没有发生变异。关键词: 江西铅山红芽芋; 胚性愈伤组织; 包埋干燥法; 超低温保存中图分类号: Q 943. 1，S 632. 3文献标识码: A文章编号: 2095－0845( 2014) 04－505－08Cryopreservation of Jiangxi Yanshan Ｒed Bud Taro ( Colocasiaesculenta var． cormosus cv． Hongyayu) EmbryogenicCalli by Encapsulation-dehydrationHONG Sen-Ｒong，YIN Ming-Hua＊＊ ，WANG Ai-Ping( College of Life Sciences，Shangrao Normal University，Shangrao 334001，China)Abstract: Cryopreservation of Jiangxi Yanshan red bud taro ( Colocasia esculenta var． cormosus cv． Hongyayu) em-bryogenic calli by encapsulation-dehydration was studied． The results showed that the optimal preculture condition of cryopreservation by encapsulation-dehydration was precultured on MS medium supplemented with 0. 75 mol·L－1 su- crose for 3 days． The optimal dehydration method was dehydration by sterile air in a laminar flow hood for 7 h or ster- ile dry silica gel for 11 h． the optimal thawing temperature was 37 ℃ ( 2 min) ． The optimal culture condition after cryopreservation was first in the dark for 7 d and then transferred to the photoperiod of 14 h·d－1 ． The average surviv- al rate of embryogenic calli after cryopreservation by encapsulation-dehydration was about 45%． Cryopreservation time and whether the removal of encapsulated calcium alginate had no significant impact on the survival rate． Mor- phological and cytological study demonstrated that the regenerants were genetically and morphological stable．Key words: Ｒed bud taro ( Colocasia esculenta var． cormosus cv． Hongyayu) ; Embryogenic callus; Encapsulation- dehydration; Cryopreservation芋具有较丰富的营养价值，在 当 今 蔬 菜 消费市场中占有重要地位，是我国南方主要的蔬菜cormosus cv． Ho