山竹果皮中抗氧化活性物质的提取分离 STUDY ON EXTRACTION AND PURIFICATION OF THE FLAVONOIDS FROM GARCINIA MANGOSTANA LPERICARP.pdfVIP

20019年6月 科学研究 食品研究与开发 第30卷第6期 ll==- 山竹果皮中抗氧化活性物质的提取分离 张泽生。赵璐。牟浩，索茂荣 (天津科技大学食品工程与生物技术学院，天津300457) 摘要：将山竹果皮95％乙醇提取物用溶剂萃取法分成4个不同的组分；采用体外抗氧化模型测定4个组分的抗氧化 能力；并对该4个组分分别测定总黄酮、总酚的含量；用大孔吸附树脂D101纯化分离正丁醇相；并测定正丁醇相纯化 前后抗氧化活性、总黄酮和总酚含量的变化。结果表明：正丁醇相表现出较强的抗氧化活性；而且该相中总黄酮和总酚 的含量也较其他几相高；用D101大孔吸附树脂纯化正丁醇相后抗氧化活性及总黄酮、总酚含量都有显著的提升。 关键词：山竹果皮；抗氧化性；总黄酮；总酚；分离；纯化 STUDYONEXTRACTIONANDPURIFICATIONOFTHEf’LAV0—0IDS FROM6诅Rc．，^ⅡH肘rAⅣGDS烈ⅣALPERICARP ZHANG Lu，MUHao，SUOMao-rong Ze-sheng，ZHAO ofFood and Biological University (CollegeEngineering Technology,Tianjin of Science＆Technology，Tianjin300457，China) Abstract：TheGarcinia L waJsextracted95％ethan01．Andtheextractsweredivided mangostanaperiearp by intofour their theirantioxidantactivitiesweredeterminedexternalantioxidant partsaccordingpolarity．Then by n—butanolextractswere mode．Besides．thecontentofflavonoidsand ofthemweredetermined．The polyphenols withadsorbresinD101．Andthenthecontentofflavonoidsand ofthemweredetermined． purified polyphenols TheresultshowedthatThen．．butanolextractshave antioxidantactivitiesandthecontentofflavonoids strong and ofitwere thantheother then-butanol showedthatthe polyphenolshigher parts．Afterpurified extracts，it of and were enhanced． antioxidantactivitiesandthecontentflavonoids