原创力文档淀粉液化芽孢杆菌产β-葡聚糖酶培养基优化以及酶稳定剂的研究 Optimization of 3-glucanase production medium with Bacillus amyloliquefaciens and 3-glucanase stabilizer.pdfVIP
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淀粉液化芽孢杆菌产β-葡聚糖酶培养基优化以及酶稳定剂的研究 Optimization of 3-glucanase production medium with Bacillus amyloliquefaciens and 3-glucanase stabilizer
2006N0．4
China
·18· SerialNo．157 Brewing Research
淀粉液化芽孢杆菌产J3一葡聚糖酶培养基优化以及
酶稳定剂的研究
郝秋娟，李永仙，李舻
(江南大学教育部工业生物技术重点实验室，江苏无锡214036)
摘要：采用正交试验方法，进行了淀粉液化芽孢杆菌发酵产B．葡聚糖酶培养基的优化。结果表明，采用玉米粉309／L：大
麦粉409／L，豆饼粉309／L，Na2HP04·12H2069／L，(NH4)2S0449m，CaCl2
0．89／L，MgS04·7H20lg／L鲑l成的培养基发酵，B．葡
酶液的残余酶活比未经处理的酶活提高了55．3％。
关键词：淀粉液化芽孢杆菌；培养基优化；B一葡聚糖酶；稳定剂
中图分类号：TQ464．8文献标识码：B 文章编号：0254—5071(2006)04—0018—05
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