海口地区胃十二指肠疾病患者幽门螺杆菌vacA基因亚型分析.docVIP

海口地区胃十二指肠疾病患者幽门螺杆菌vacA基因亚型分析 第38卷第5期第692页 2009年10月 华中科技大学(医学版) ActaMedUnivSciTechnolHuazhong Vo1.38No.5P.692 Oct.2009 海口地区胃十二指肠疾病患者幽门螺杆菌 vacA基因亚型分析* 汤净陈军王丹妹曾仕平王志英周元平△ 海南医学院临床系,海口570102 .南方医科大学南方医院感染科,广州510515 摘要目的探讨幽门螺杆菌(H.pylori)vacA基因亚型在海口地区胃十二指肠疾病患者中的分布.方法从176 例胃十二指肠疾病患者胃黏膜标本中分离培养H.pylori,PCR法测定vacA基因亚型.结果176例患者中的基因型 vacAsl78.98(139/176),vacAs215.34(27/176),vacAml28.41(50/176),vacAm271.59(126/176);vacA亚型 组合形式为:vacAsl/ml28.41(50/176),vacAsl/m256.25(99/176),vacAs2/m215.34(27/176),未发现va— cAs2/ml型菌株;消化性溃疡,胃癌,慢性萎缩性胃炎患者H.pylori的vacAsl,vacAm2,vacAsl/m2基因型阳性率高于 慢性浅表性胃炎患者(均Plt;O.O1),其余基因型在不同类型疾病中的分布无统计学差异(Pgt;O.05).结论海口地区胃 十二指肠疾病患者H.pylori菌株的优势基因型为vacAsl,vacAm2,vacAsl/m2;在消化性溃疡等严重疾病患者中多见. 关键词幽门螺杆菌;细胞空泡毒素基因;胃十二指肠疾病 中图法分类号R378.2DOI:10.3870/j.issn.1672—0741.2009.05.030 AnalysisofvaeASubgenotypesofHelicobacterpyloriinPatients withGastrOdu0denaIDiseasesinHaikou TangJing,ChenJun,WangDanmeietal DepartmentoyGastroenterology,HainanMedicalCollege,Haikou570102 AbstractObjectiveToinvestigatethedistributionofpredominantvacAsubgenotypesofHelicobacterpylori(H.pylori) ingastr0du0denaldiseasesinHaikouandstudytherelationshipbetweenvacAsubgenotypesinH.声ylori—associateddisea— ses.MethodsVacAsubgenotypesof176H.pyloristrainsisolatedfrompatientswithchronicgastritis.pepticulcer.chronica— trophicgastritisandgastriccancerweredetectedbypolymerasechainreaction(PCR).ResultsThepositiverateofthevacAsl. vacAs2,vacAml,vacAm2andvacAsl/m2genotypesofl76H.pyloristrainswas78.98(139/176),15.34%(27/176), 28.41(50/176),71.59(126/176)and56.25(99/176)respectively.ThevacAs2/mltypewasnotfound.Thepositiverate ofvacAsl,vacAm2andvacAsl/m2genotypeswashigherinpatientswithpepticulcer,chronicatrophicgastritisandgastric cancerthaninthosewithchronicgastritis(Pd0.01).Therewasnodifferenceinothergenotypesamongdifferentgastricduode na1diseases.ConclusionVacAs1.vacAm2andvacAsl/m2arepredominantgenotypesofH.PyloristrainsisolatedfromHaik— oupatmnts,andthesegenotypesaredispersedinpepticulcer,chronicatrophicgastritisandgastriccancer?