脐带间充质干细胞对神经元的修复及重建.doc

脐带间充质干细胞对神经元的修复及重建 沈大鹏，王炎 摘要 目的：观察脐带间充质干细胞移植对帕金森病神经元细胞的修复与重建。 方法：选择2011—01／12枣庄市妇幼保健院与华北油田总院收治的帕金森病患者30例，均经过英国皇家医师学会帕金森病诊断与管理指南的帕金森病诊断标准确诊，男16例，女14例，年龄45-66岁，平均年龄56岁，Hoehn—Yahr分级为II一Ⅳ级。按患者意愿，30例帕金森病患者分为细胞移植组、对照组，15例/组，两组患者在性别、年龄、UPDRS评分运动部分、日常生活能力评分方面比较均无显著性差异(P0．05)。所有患者均住院治疗，第1周行常规检查，自第2周起移植组患者应用脐带间充质干细胞进行鞘内注射移植治疗， 1次／周，4次为1个疗程，共治疗1个疗程。治疗组患者采用常规复方左旋多巴制剂进行药物治疗。采用帕金森病统一评分量表对患者治疗前后神经功能进行评定，分值越高表示神经功能缺损越严重。移植组细胞移植前及移植后4周，12周分别行中脑黑质磁共振破谱分析。用配套软件测量各峰下面积并分别计算NA/Cr、Cho／Cr和NAA／Cho的比值。比值越高提示神经元修复越好。 结果：移植组UPDRS总分和UPDRSIII评分均值显著低于治疗组(P0．01)，UPDRSⅡ评分均值低于治疗组且有统计学意义(P0．05)。移植组患者移植后其症状对侧黑质NAA／Cr，NAA／Cho较移植前的相应比值均值明显下降，两组差异有显著意义(P0．05)。 结论：脐血间充质干细胞移植对多巴胺能神经元有修复与再生作用，可以一定程度地改善帕金森病患者的临床症状。 关键词：脐带间充质干细胞；神经元的修复与再生，细胞移植；帕金森病； Using umbilical cord blood mesenchymal stem cell transpl- -antation in repairing and regenerating of neurons Dapeng Shen Yan Wang Abstract Objective：To observe the curative effect of umbilical cord mesenchymal stem cells (UC-MSCs) transplantation in reparing and reconstruacting for neurons of Parkinson’s disease .(PD)METHODS: A total of 30 PD patients admitted in the Maternal and Child Health Hospital of Zaozhuang City,and General Hospital of Huabei Oilfield from January to December 2011, were selected, including 16 males and 14 females, aged 45-66 years. Average age of 56 years. Hoehn-Yahr grade II一.The subjects were in accordance with diagnostic criteria of UK Parkinson’s disease society Brian Bank Clinical Diagnostic Criteria . The patients were equalIy divided into cell transplantation and treatment groups according to patients’will. 15 cases / group , the two groups were compared in the gender,age , moving parts of UPDRS score , and activity of daily lifing, the difference was not significant (P 0.05). All patients were hospitalized, Routine examination in first week. From the second week, the cell transplantation patients received UC-MSCs implantation by intrathecal injection , 1 times / week , 4 times for a course of treatment Were treated with a course