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Microwave-assisted Extraction Used for the Isolation of Total Flavonoids from Cyclocarya paliurus (Key Laboratory of Food Science of Ministry of Education, Nanchang University, Nanchang 330047, People’s Republic of China) Abstract: Microwave-assisted extraction (MAE) of total flavonoids from Cyclocarya paliurus was studied.Response surface methodology was employed to optimize the parameters of the Microwave-assisted extraction on the yield of total flavonoids which were directly determined by colorimetric method without any further treatment.Four independent variables were temperature , extraction time (min),solid:liquid ratio,the concentration of alcohol.Considering the feasibility of experiment, the optimum conditions of extraction were temperature 77 centigrade, extraction time 15min, solid:liquid ratio 1:21,the concentration of alcohol 63%.The predicted values in the optimal condition was 16.183499mg/g and had a close agreement to experiment value.MAE has many advantages, such as simple,shorter time,less solvent and higher extraction rate,which can be used to extract total flavonoids from Cyclocarya paliurus. Keywords: Cyclocarya paliurus; microwave-assisted extraction; total flavonoids; response surface methodology Introduction Cyclocarya paliurus (Batal.) Iljinskaja (C. paliurus) is a Juglandaceae plant, which is widely distributed in southern China. C. paliurus is the sole species in its genus ,which is mainly found at _______________________________ * Corresponding author: Ming-Yong Xie, Fax: +86 791-8305826 E-mail address: myxie@ncu.edu.cn the humid and fertile high mountainous regions of Jiangxi,Zhejiang,Jiangsu,Anhui,Fujian,Taiwan, Hubei,Sichuan,and Guizhou province. 青钱柳化学成和生物活性研究概况C. paliurus is a medicinal herb, which has been widely used in China both as d