杭州市两起诺如病毒GⅡ17型暴发疫情分子流行病学特征.docVIP

杭州市两起诺如病毒GⅡ17型暴发疫情分子流行病学特征 陈彬彬 潘峰 孙昼 杭州师范大学附属医院 杭州市疾病预防控制中心 X 关注成功！ 加关注后您将方便地在 我的关注中得到本文献的被引频次变化的通知！ 新浪微博 腾讯微博 人人网 开心网 豆瓣网 网易微博 摘????要： 目的 对杭州市两起诺如病毒感染暴发疫情开展调查及病原分子鉴定分型。方法 对两起暴发疫情开展流行病学调查, 采集病人肛拭子标本及外环境标本进行诺如病毒GⅠ/GⅡ型检测, 对阳性标本的病毒基因VP1区部分核酸序列进行扩增测序, 利用生物信息学软件进行多序列比对排列及构建进化树。结果两起疫情分别发生在残障人士福利院及农村中学, 报告病例数为54例及161例, 感染原因分别为接触传播及桶装水污染传播。采集29人份病人肛拭子标本、5份桶装水标本和10份食品及涂抹物标本, 结果19人份肛拭子标本诺如病毒阳性, 对其中6份阳性标本进行序列比对及进化分析, 病毒株均为诺如病毒GⅡ17型, 与2014年美国株 (KY424350) 、2014年中国湖北株 (KU310887) 、2015年中国台湾株 (KT732275) 同源性高。结论 两起暴发疫情均由诺如病毒引起, 基因型别为GⅡ17型。 关键词： 诺如病毒; 急性胃肠炎; 暴发疫情; 基因型; 作者简介：陈彬彬, 本科, 主管技师, 主要从事分子生物学和临床检验工作 作者简介：孙昼, E-mail:hzcdc@ 收稿日期：2017-06-12 Molecular epidemiological characteristics of two outbreaks caused by norovirus GⅡ17 genotype during December 2014 and January 2015 in Hangzhou, China CHEN Bin-bin PAN Feng SUN Zhou The Affiliated Hospital of Hangzhou Normal University; Abstract： Objective To summarize the epidemiologic and the molecular characteristics of the two outbreaks caused by norovirus in Hangzhou of China from December 2014 to January 2015. Methods The epidemiological survey was conducted and the anal swabs and environmental samples were collected to detect GⅠ/GⅡ norovirus nucleic acids.The VP1 region nucleic acid sequence of positive strains were amplified and sequenced.The genotype of the isolates were compared and the phylogenetic tree was constructed by bioinformatics software. Results The two outbreaks occurred in a welfare center and a rural school.The number of reported cases were 54 and 161.Contact transmission and drinking contaminated barreled pure water may cause the outbreaks.The 29 anal swabs of the patients, 5 samples of barreled water and 10 samples of food and smear on the object surface were collected.The 19 anal swabs were positive for norovirus nucleic acids.The homologous and phylogenetic analysis showed that the isolates belonged to norovirus type GⅡ17.These strains show high homology in sequences of both nucleotides and amino acids