乳铁蛋白免疫增强作用评价.pdfVIP

244 2010, Vol. 31, No. 11 食品科学 ※营养卫生 胡志和，李 娜，刘传国，庞广昌，陈庆森 (天津市食品生物技术重点实验室，天津商业大学生物技术与食品科学学院，天津 300134) ：根据我国免疫调节功能食品的评价方法，对乳铁蛋白的免疫调节作用进行评价。在实验中，采用Balb/c 小鼠建立免疫低下模型，以不同剂量(100 μg/mL 、1mg/mL、10mg/mL)乳铁蛋白灌胃实验动物，分别进行免疫脏器 质量、细胞免疫功能、体液免疫功能、单核- 巨噬细胞功能指标的检测。结果表明，在灌胃剂量为0.1～10mg/mL 范围内，结果均为阳性，且以1mg/mL 最为显著。判定乳铁蛋白具有增强免疫力，但与剂量有关。 ：乳铁蛋白；免疫增强；灌胃 Evaluation on Immune-enhancement Effect of Lactoferrin HU Zhi-he ，LI Na ，LIU Chuan-guo ，PANG Guang-chang ，CHEN Qing-sen (Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China) Abstract：The immune regulatory function of lactoferrin was evaluated according to relevant Chinese national standards for evaluation of immune regulatory functional food. Balb/c mice were chosen to establish an immunodeficiency model. The immunodeficiency mice were orally administered lactoferrin at the dosages of 0.1, 1 mg/mL and 10 mg/mL, respectively. The effect of lactoferrin on immune organ weight, cellular immune function, humoral immune function and monocyte macrophages function were determined. The results showed that lactoferrin exhibited positive reaction within the dosage range of 0.1 －10 mg/mL, and the strongest immune effect of lactoferrin was 1 mg/mL. Therefore, it can be concluded that lactoferrin exerts immune regulatory function in a dose-dependent manner. Key words ：lactoferrin ；immune enhancement ；intragastric administration 中图分类号：R151.2 文献标识码：A 文章编号：1002-6630(2010)11-0244-05 牛乳铁蛋白(bovine lactoferrin ，BLF)是一种分子质 抗氧化剂、营养强化剂、免疫增强剂等，并将其应用 量为80kD 的铁结合糖蛋白，1939 年在牛乳中被发现， 于保健食品、婴幼儿食品、化装品、药品等领域[ 2 7 ] 。 1960 年被定义为乳铁蛋白。从20 世纪80 年代开始规模