宫颈疾病高危型HPV感染者核酸杂交检测结果分析.docVIP

精品论文 参考文献 宫颈疾病高危型HPV感染者核酸杂交检测结果分析 佴科军 夏丽梅 乔丽华 包兴锋 陆仕洪 张炜 　　(上海市第一人民医院分院 200081) 　　【摘要】目的 了解宫颈疾病人乳头状瘤病毒（HPV）感染患者中HPV亚型分布情况及高危型HPV的年龄分布。方法 采用专利Invader酶切信号放大法，利用Cervista HPV HR Cervisat核酸杂交基因分型试剂检测宫颈脱落细胞HPV的14个亚型DNA。结果 280例宫颈疾病患者，核酸分子杂交检测结果显示，104例感染一种或两种以上的高危型HPV，感染率37.14%，其一种HPV亚型感染率30.00%，混合感染的感染率则为7.14%；宫颈疾病HPV感染者中以高危型HPV 16、43、58、33和6型感染位列前五位；在不同年龄组中以20-50岁组HPV感染率最高。结论 本地区宫颈疾病中高危型HPV感染以16、43、58、33和6型单一亚型感染最为常见，且以青壮年为主。 　　【关键词】核酸杂交 人乳头状瘤病毒 宫颈 　　【中图分类号】R713 【文献标识码】A 【文章编号】2095-1752（2014）12-0040-02 　　Analysis of detection of nucleic acid hybridization in high risk human papilloma viral infection patients of cervix disease 　　Nai Kejun, Xia Limei, Qiao Lihua 　　The Branch of First People Hospital in Shanghai City. 200081 　　【Abstract】 Objective To comprehend subtype and age distribution of high risk human papilloma viral infection in cervix disease patients. Method Signal amplification method of patent Invader enzyme digestion was adopted. The 14 HPV subtypes DNA were detected by Cervista? HPV HR Cervisat kit of nucleic acid hybridization. Results In 280 cervix disease patients, the results showed that 104 patients were one and above two HPV subtype infection by hybridization of nucleic acid. The infection rate was 37.14%. Infection rate of single HPV subtype was 30%, but mixed infection rate was 7.14%. HPV 16, 43, 58, 33 and 6 subtypes and mixed HPV were ranked in front fifth of HPV infection patients. Infection rate was the highest in from 20 to 50 years compared with other groups. Conclusion The 16, 43, 58, 33 and 6 subtyps of high risk single HPV and mixed HPV infection were most common. The HPV infection rate was higher in from young and strong population. 　　【Key words】 nucleic acid hybridization human papilloma viral cervix 　　人乳头状瘤病毒（human papillomavirus，HPV）感染是宫颈病变的主要病因，有研究显示,目前已发现HPV 亚型中约有40个与生殖道病变有关，几乎所有宫颈癌患者可检测到高危型HPV DNA[1]。HPV 感染的亚型存在地域差异，全世界以HPV16、18型感染率最高，而亚洲常见58、52型，也有关文献报道，某些地区高危型HPV