Survivin基因真核表达载体的构建及鉴定.pdfVIP

Survivin基因真核表达载体的构建及鉴定 范红杰1于维东何志义3 中国医科大学1.附属盛京医院神经内科，沈阳1100042.附属第一医院干诊科，沈阳1100013.附属第一医院神经内科，沈阳110001 目的 构建pcDNA3.1-Survivin基因的真核表达载体并鉴定。方法 提取大鼠肝细胞总RNA，RT-PCR法扩增Survivin cDNA片段，双酶切构建pcDNA3.1-Survivin，重组载体。结果 重组真核表达载体经PCR、酶切鉴定及测序证实正确。结论 成 功构建了真核表达载体pcDNA3.1-Survivin。 Survivin基因；构建；鉴定 R737.33 A 0258-4646( 2012)02-0128-03 Construction and Identification of Survivin Gene Eukaryotic Expression Vector FAN Hong-jie YU Wei-dong HE Zhi-yi 辽宁省教育厅高校科研计划（05L485） 范红杰（1965-）女，副教授，博士. E-mail: fanhongjieonly@ 163.com 2011-11-23 经BLAST 突变无移 @@[ 1 ] Van Houdt UJ,Haviv YS,Lu B,et al. The human survivin promoter:a novel transcriptional targeting strategy for treatment of glioma [J]. J Neurosurg, 2006,104(4) :583-592. @@[2] Conway EM,Pollefeyt S,Comelissen J,et al. Three differentially ex pressed survivin cDNA variant s encode proteins with distinct anti apoptotic functions [J]. Blood,2000,95 ( 4): 1435. @@[ 3 ] Pennati M, Folini M ,Zaffaroni N. Targeting survivin in cancer thera py[J]. Expert Opin Ther Targets,2008,12 (4):463-476. @@[4] Shi Y. Survivin structure:crystal unclear [J]. Nat Struct Biol,2000, 7 ( 8 ) : 620-623. @@[5] Mesri M,Wall NR,Li J,et al. Cancer gene therapy using a survivin mutant adenovirus [J]. J Clin Inv,2001,108 ( 7):981-990. @@[6] Grossman D,Kim PJ,Blanc Brude OP,et al. Taansgenic expression of survivin in keratinocytes counteract s UVB-induced apoptosis and cooperates wit h loss of p53 [J ]. J Clin Inv,2001,108 (7) :991-999. @@[7]范红杰，王宗立，何志义.大鼠脑缺血再灌注损伤后Survivin和 Caspase-3表达与细胞凋亡的关系[J].中国现代医学杂志，2010， 20(20)：3065-3073. @@[8 ] LI W, Ma N ,Ong L,et al.Bcl-2 Engineered MSCs inhibited apoptosis and improved heart function [J ]. Stem Cells,2007,25 (8) :2118- 2127.