脑源性神经营养因子在母牛性腺及性腺外生殖组织中的表达.PDFVIP

2010, 32(4) :443~ 446 htt :/ / xuebao.jlau. edu. cn Journal of Jilin Agricultural University E2mail :jlndxb@vi . sina. com X XX 1, 2 1 1 1, 3 1 1 孙永峰 , 李纯锦 , 马永和 , 王春强 , 张 伟 , 周 虚 1. , 130062; 2. , 130118; 31 , 121001 : 采用RT- PCR 方法及免疫组织化学技术检测脑 性神经长生因子BDNF 基因在母牛卵泡期和黄体 期的卵巢、输卵管和子宫组织中的表达和定位情况。结果表明: BDNF mRNA 表达于上述2 个时期 3 种组织 中, 主要表达于卵巢的颗粒细胞和膜细胞、输卵管的黏膜上皮细胞、子宫内膜上皮细胞及子宫腺细胞, 且各组 织间免疫活性的表达强度明显不同。 : 脑 性神经营养因子; 表达; 性腺; 牛 : S82313; Q786 : A : 10002 5684(2010) 042 04432 04 Expr ession of Br ain2Derived Neur otr ophic Factor in Gonad an d Ext ernal Repr oductive Tissues of Cow 1, 2 1 1 1, 3 1 SUN Yong2 feng , LI Chun2jin , MA Yong2he , WANG Chun2qian g , ZHANG Wei , ZHOU 1 Xu 1. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China ; 2. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, Chi2 na; 3. College of Animal Science and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, China Abstract: In t e experiment, by RT- PCR and immuno istoc emistry, t e expression and location of BDNF mRNA in ovary, oviduct and uterus from luteal p ase and follicular p ase ovary of cattle were de2 tected. T e results s owed t at t e mRNA and protein of BDNF were present in t e above t ree tissues of two periods, mainly expressed in granular cell and t eca cell o