硫链丝菌肽抑制FOXM1表达后对裸鼠鼻咽癌移植瘤的抑制作用.docVIP

硫链丝菌肽抑制FOXM1表达后对裸鼠鼻咽癌移植瘤的抑制作用 于 超,2，李艮平3，陈鸿雁2 （400021重庆，，，外科研究所）摘要 目的方法kg)；观察各组裸鼠移植瘤的生长情况，绘制生长曲线，最后瘤体称重计算抑瘤率；成瘤后取瘤体组织制成细胞悬液，流式细胞法检测细胞周期变化及细胞凋亡发生情况；提取瘤体组织蛋白进行Western blot检测结果结论硫链丝菌肽可以FoxM1表达，有效鼻咽癌CNE-2细胞关键词 鼻咽；FoxM1；硫链丝菌肽； [中图法分类号R766.3 [文献标志码Inhibitive effect of TST on human nasopharyngeal Carcinoma (CNE-2 cell line) transplanted in nude mice Yu Chao1,2,Li Genpin3,Chen Hongyan2 (1Department of Otolaryngology ，Chongqing Traditional Chinese Medicine Hospital, Chongqing, 400021; 2Department of Otolaryngology Head and Neck Surgery, First affiliated hospital, Chongqing Medical University, Chongqing, 400016;3Dapin Hospital ,Research Institute of Surgery, Third Military Medcical University, Chongqing,400042,China) [Abstract] Objective To investive the inhibitive effect of TST on human nasopharyngeal Carcinoma (CNE-2 cell line) transplanted in nude mice. Methods CNE-2 cancer cells were xenografted into nude mice which were randomly divided into 2 groups:control group.TST treatment group(200μg/kg).Tumor growth was observed the mice were weight,and the tumor inhibition rate was caculated.The apoptosis rate and cell cycle was analized by flow cytometry. Expression of FoxM1 protein and apoptosis proteins were detected by western blot. Results The mean tumor volume in TST treatment group was significantly lower than that in the control group(P＜0.05).The tumor weight :TST group:(0.582 ±0.172)g,control group:(1.118 ±0.159)g，The tumor inhibition rate of the TST treatment group was 47.94%.Flow cytometry assay indicated that the apoptosis rate and the percentage of G1 phase cell numbers were both increased in TST treatment group.Western blot showed that the expression levels of FoxM1 protein and apoptosis associated proteins cyclin-D1，skp2 decreased ,P27 protein increased in TST treatment group. Conclusion TST can effectively inhibit human nasopharyngeal Carcinoma (CNE-2 cell line) transplanted in nude mice. [Key words] nasopharyngeal carcin