白茶寿眉预防小鼠肥胖作用研究及安全性评价.docVIP

白茶寿眉预防小鼠肥胖作用研究及安全性评价 郑丽 侯彩云 任发政 北京食品营养与人类健康高精尖创新中心食品科学与营养工程学院中国农业大学 食品质量与安全北京实验室食品科学与营养工程学院中国农业大学 X 关注成功！ 加关注后您将方便地在 我的关注中得到本文献的被引频次变化的通知！ 新浪微博 腾讯微博 人人网 开心网 豆瓣网 网易微博 摘????要： 取1、3、7年寿眉 (白茶) 制备白茶提取物 (White tea extracts, WTEs) 。在对雄性C57BL/6J小鼠喂饲高脂饲料的同时给予WTEs干预, 诱导建立预肥胖模型。观察小鼠体质量增量、摄食量、脂肪和肝脏组织病理学变化, 统计脂肪湿重, 测定血脂及血转氨酶水平。q PCR测定小鼠肝脏中脂代谢相关基因表达。综合评价WTEs预防小鼠肥胖功效及其安全性, 并对其机制进行探究。结果显示, WTEs在无肝毒性和生长抑制作用条件下, 能抑制脂肪细胞分化和脂肪的过度积累, 还能下调SREBP-1c、FAS和ACC1的转录水平, 抑制肝脏脂肪酸合成和脂肪过度积累, 有效预防肥胖和脂肪肝的发生。高剂量下, WTEs预防肥胖功效随白茶年份的增加而显著降低。 关键词： 白茶; 预防肥胖; 安全性; 作者简介：郑丽, 女, 硕士研究生, 主要从事白茶预防肥胖功能性评价等研究, E-mail163.com。 作者简介：侯彩云 food319@139.com 收稿日期：2017-03-23 基金：北京食品营养与人类健康高精尖创新中心开放基金项目 Anti-obesity Efficacy of Shoumei White Tea on High-fat Diet Mice and Its Safety Evaluation ZHENG Li HOU Caiyun REN Fazheng Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University; Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University; Abstract： White tea extracts (WTEs) had been prepared from Shoumei white tea stored for 1, 3 and 7 years, respectively. Male mice (C57 BL/6 J) were fed with high-fat diet and supplemented with WTE to establish the anti-obesity efficacy model. Body weight gain, food intake, the pathobiology changes of the liver and adipose tissues, serum lipids profiles and differential expression profiles of lipid metabolism-related genes of liver in mice were investigated. The results showed that WTEs inhibited adipocyte differentiation and lipid excessive accumulation in adipose tissues, inhibited fatty acid synthesis and lipid excessive accumulation in liver by ameliorating the expression levels of lipid metabolism-related genes via down-regulation of the m RNA expression level of SREBP-1 c, FAS and ACC1, thus depressing the increase of body weight and adipose tissues induced by high-fat diet. The preventing o