纳米氧化石墨烯颗粒对小鼠心肌酶的影响.docVIP

纳米氧化石墨烯颗粒对小鼠心肌酶的影响 刘艳 岳美 马荣爽 陈杰 于杨 张睿智 赵淑华 内蒙古医科大学公共卫生学院环境与职业卫生学教研室 吉林大学公共卫生学院环境卫生与劳动卫生学教研室 X 关注成功！ 加关注后您将方便地在 我的关注中得到本文献的被引频次变化的通知！ 新浪微博 腾讯微博 人人网 开心网 豆瓣网 网易微博 摘????要： 目的 探讨一次性尾静脉注射纳米氧化石墨烯颗粒 (GO) 对小鼠心肌ATP酶、LDH、AST活性影响, 为其在生物学领域的安全应用提供科学依据。方法 选择80只清洁级ICR小鼠, 体重 (18±2) g, 雌雄各半, 随机分为4组, 分别以剂量为1.40、0.70、0.35 mg/kg氧化石墨烯对小鼠一次性尾静脉注射, 对照组注射蒸馏水。并于注射后3 d和15 d将小鼠脱臼处死, 取心脏, 测定心肌匀浆液中的Na+, K+-ATP酶、Ca2+, Mg2+-ATP酶、LDH、AST活性测定。结果 注射后3 d, 雌、雄中、高剂量Na+, K+-ATP酶活性及Ca2+, Mg2+-ATP酶活性均高于对照组 (P0.05) 。15 d后, 雌、雄各剂量组Na+, K+-ATP酶及Ca2+, Mg2+-ATP酶活性均低于对照组 (P0.05) 。注射后3 d, 雌、雄各组间AST活性无统计学意义 (P0.05) , 15 d后, 雌、雄各剂量GO组明显低于对照组 (P0.05) 。注射3 d, 雄性各剂量GO组LDH活性明显低于对照组 (P0.05) , 雌性高剂量GO组明显低于对照组和低剂量GO组。注射后15 d, 雄性高剂量GO组显著低于中、低剂量GO组 (P0.05) , 雌性中、高剂GO量组明显低于对照、低剂量GO组 (P0.05) 。结论 一次性尾静脉注射氧化石墨烯可干扰Na+, K+-ATP酶、Ca2+, Mg2+-ATP酶和AST、LDH等心肌酶活性的作用。 关键词： 氧化石墨烯; 钠钾ATP酶; 钙镁ATP酶; 乳酸脱氢酶; 谷草转氨酶; 作者简介：刘艳 (1990-) , 硕士, 助教, 从事教学工作。 作者简介：赵淑华, E-mail:Shzhao@jlu.edu.cn 收稿日期：2017-04-15 Influence on the ATPase, LDH and AST activity of mice myocardium after injected the Graphene oxideintravenously for once LIU Yan YUE Mei MA Rong-shuang Chen Jie Yu Yang Zhao Shu-hua Department of Environmental and Occupational Hygiene of School of Public Health, Inner Mongolia Medical University; Abstract： Objective Discuss the influence on ATPase, LDH, AST activity of myocardium of mice after they were injected for GO.Methods With the dose of 1. 40, 0. 70, 0. 35 mg/kg graphene oxide on mouse intravenous injection, the control dose group was injected with distilled water, and mice were put to death on Day 3 and Day 15 after injection, myocardial tissue homogenate and the activity of Na+, K+-atpase, Ca2+, Mg2+-atpase, LDH and AST were detected.Results On Day 3 after injection, the activity of Na+, K+-atpase, Ca2+, Mg2+-atpase in middle and high dose groups were higher than those in control group; both in male and female; On Day 15 after injection, the activity of Na+, K+-atpase, Ca2+, Mg2+-atpase in each dose group were higher than