浅析2011～2013年大庆市手足口病标本病毒检测结果分析.docVIP

精品论文 参考文献 浅析2011～2013年大庆市手足口病标本病毒检测结果分析 黑龙江省大庆市红岗区杏树岗镇卫生院 163512 　　【摘 要】目的 对2011～2013年大庆市手足口病病例的不同标本进行病原体检测和分析，了解到大庆市的手足口病感染状况。方法 对临床诊断为手足口病的患者的粪便标本460份，咽拭子标本549份（EV阳性345份，EV71阳性23份，CA16阳性13份），用RT-PCR是实时荧光定量PCR检测肠道通用病毒、CVA16和EV71，华山的急性期血清采用酶联免疫吸附试验检测EV71和CAV16的IgM抗体。结果 患者的粪便标本肠道病毒总阳性率为82.26%（378/460），CVA16的阳性率为37.40%（172/460），EV71的阳性率为25.41%（116/460），其他肠道病毒为19.66%（90/460），CVA16行阳性率高于EV71。粪便标本的肠道病毒阳性率高于咽拭子的38.75%（345/549）（chi;2=27.01，Plt;0.01）。发病年龄多为1～5岁，主要集中在3岁左右，男性患者多余女性患者。对手足口病病例的急性期血清进行酶免疫检测，EV71和CVA16的阳性率分别为28.70%（31/108）和33.33%（36/108），和粪便标本的阳性率的核酸检测比较，有较高的相关性。结论 2011～2013年度本市的手足口病疫情以肠道病毒通用型为主，粪便标本的阳性率较高，急性期血清的免疫实验结果和分子生物系相符，采用分子生物学方法检测手足口病的病原体，用酶联免疫吸附试验检测血清抗体，对手足口病的诊断有重要作用。 　【关键词】手足口病；肠道病毒；EV71；CA16 　Abstract：the pathogen detection and analysis of different samples of 2011 ~ 2013 in HFMD cases，learned that the city of HFMD infection. Methods the clinical diagnosis of stool specimens of HFMD patients in 460 samples，549 samples of pharynx swabs（345 were EV positive，23 were EV71 positive，13 were positive for CA16），with RT-PCR real time fluorescence quantitative PCR detection of enterovirus，CVA16 and EV71，Huashans acute phase serum by ELISA the adsorption of IgM antibody test for the detection of EV71 and CAV16. The stool specimens of the patients with intestinal virus positive rate was 82.26%（378/460），the positive rate of CVA16 was 37.40%（172/460），the positive rate of EV71 was 25.41%（116/460）and other enterovirus 19.66%（90/460），CVA16 positive rate was higher than EV71. The positive rate of enterovirus stool specimens is higher than that of pharyngeal swab 38.75%（345/549）（2=27.01，Plt;0.01）. The age of onset was 1 to 5 years old，mainly concentrated in the age of 3 years old，male patients with excess female patients. On foot and mouth disease cases in acute phase serum by enzyme immunoassay，the positive rate of EV71 and CVA16 were 28.70%（31/108）and 33.33%（36/108），comparison of nucleic acid detection and positive rate of stool specimens，had a higher correlation.