湿式与干式大肠菌群快检纸片的应用研究.docVIP

精品论文 参考文献 湿式与干式大肠菌群快检纸片的应用研究 刘德鹏 郝英敏 姜玉芳 　　（山东省莱州市疾病预防控制中心 山东 莱州 261400） 　　【摘要】 目的：提供一种生产成本低、使用方便、结果准确的湿式大肠菌群快检纸片。方法：对依据“食（饮）具用大肠菌群快速检验纸片质量标准”要求加工生产的湿式大肠菌群快检纸片与干式大肠菌群快检纸片进行质量检测和对比试验。结果：湿式大肠菌群快检纸片与干式大肠菌群快检纸片的质量均符合“食（饮）具用大肠菌群快速检验纸片质量标准”的规定要求，经过400份食（饮）具的检测对比试验，两种快检纸片检测的阳性符合率为97.2%(69/71),阴性符合率为99.4%(329/331),总符合率为99.5%(398/400),说明两种快检纸片的检测结果一致。结论：湿式大肠菌群快检纸片与干式大肠菌群快检纸片相比具有以下优点：易制备；经济成本低；使用方便，操作时省时省力，可减少或避免因加盐水湿润纸片过程中被空气污染引起假阳性的机会；该产品的湿度一致，可减少或避免因加盐水多或少而引起的结果不易判断或结果不一致；是一种具有经济效益和社会效益的成品培养基，完全可以替代干式大肠菌群快检纸片，为消毒食（饮）具中大肠菌群的检测方法的进一步完善提供了科学依据。 　　【关键词】 湿式；干式；大肠菌群快检纸片；成品培养基 　　【中图分类号】R446 【文献标识码】A 【文章编号】2095-1752（2015）28-0340-02 　　 　　【Abstract】Purpose To provide the Wet Coliform quick check paper with a low production cost, easy to use, accurate results.Methods Quality testing and comparison testing between the Wet Coliform quick check paper and Dry Coliform quick check paper which request processing production based on dinner and drinking set with coliform rapid test paper quality standards.Result The Quality of the Wet Coliform quick check paper and Dry Coliform quick check paper both meet the requirements of dinner and drinking set with coliform rapid test paper quality standards.The detection comparison test after 400 dinner and drinking sets ,the positive coincidence rates of the two kind of quick check paper were 97.2%(69/71),the negative coincidence rates were 99.4%(329/331),the total coincidence rates were 99.5%(398/400),witch means the test results of the two kind of quick check paper were consistent.Conclusion The Wet Coliform quick check paper compare with the Dry Coliform quick check paper has the following advantages:easy preparation;Low economic cost;easy to use,operating convenient,can reduce or avoide the chance of false positives caused by the air pollution due to the process of weting the quick check paper .The moisture of the product are consistent,can reduce or avoide the results are hard to judge or inconsisten