猪来源抗胸腺细胞球蛋白对食蟹猴免疫功能影响.docVIP

精品论文 参考文献 猪来源抗胸腺细胞球蛋白对食蟹猴免疫功能影响 赵越 王筱啸 钟山 向莹 陈刚（通讯作者） （华中科技大学同济医学院附属同济医院器官移植研究所 430030） 【摘要】目的 研究猪来源抗胸腺细胞球蛋白（ATG）对食蟹猴免疫功能的影响。 方法 ATG倍比稀释与食蟹猴淋巴细胞孵育，流式检测细胞毒；ATG单纯给药组分为低剂量组（250mg/日）及高剂量组（750mg/日），分别连续给药5日，并于定期检测血常规以及用流式细胞仪检测CD3/CD20、CD4/CD8细胞的比例。结果 ATG对食蟹猴淋巴细胞具有很强的杀伤作用，滴度为1：2时杀伤率为84.95%，直至滴度为1:512时才降至10%以下；给药期间体内单纯给药高剂量组与低剂量组对总淋巴细胞清除率无明显差别，但各组对CD8+淋巴细胞杀伤作用均强于CD4+淋巴细胞（Plt;0.01）。结论 ATG可以杀伤食蟹猴淋巴细胞，其中对CD8+淋巴细胞效果更显著。 【关键词】抗T细胞球蛋白 食蟹猴 同种异体肾移植 淋巴细胞 【中图分类号】R39 【文献标识码】A 【文章编号】2095-1752（2014）07-0222-02 The influence of Porcine derived anti thymocyte globulin in cynomolgus immune system ZHAO Yue1, WANG Xiaoxiao1, ZHONG Shan1，XIANG Ying1，CHEN Gang1 (1. Institute of Organ Transplantation, Tongji Hospital, Tongji Medical Collage, Huazhong University of Science and Technology, Wuhan 430030) 【Abstract】Objective To investigate the influence of ATG on cynomolgus immune function. Methods Cynomolgus lymphocytes were incubated with various concentrations of ATG to detect cytotoxicity by flow cytometry; In ATG intravenous infusion experiment, cynomolgus are divided into two groups: low dosage group (250mg/day) or high dosage group (750mg/day) and recived intravenous infusion for 5 days. Blood samples were collected for blood routine analysis and CD3, CD20, CD8 and CD4 percentage by flow cytometry. Results ATG has strong cytotoxicity effect against cynomolgus lymphocyte. When ATG titer was 1:2, the lysis rate was 84.95%, and the lysis rate dropped below 10% when the titer reached 1:512. There is no difference in in vivo ATG intravenous infusion experiment between High dosage group and low dosage group in general depletion rate during administration period. The CD8 positive cells depletion rate is stronger than the CD4 positive cells depletion rate. Conclusion ATG has a cytotoxity effect against cynomolgus lymphocytes. 【Key words】antithymocyte globulin cynomo