玉郎伞水提物对大鼠的长期毒性研究.docVIP

精品论文 参考文献 玉郎伞水提物对大鼠的长期毒性研究 施之 陈春霞 黄仁彬（通讯作者) 　　（广西医科大学药学院 广西 南宁 530021） 　　【摘要】 目的：观察玉郎伞水提物对大鼠的长期毒性，为临床安全用药提供依据。方法：80只Wistar大鼠（雌雄各半）随机等分为对照组和玉郎伞水提物低、中、高剂量组（20、40、80 g生药?kg-1?d-1，相当于人临床日拟用量的20、40、80倍），连续灌胃给药91天，停药后观察14天，对照组给以等体积蒸馏水。给药期及停药恢复期，观察大鼠的体征、行为、摄食量、排泄等一般状况，及血液学、血液生化指标、脏体系数、病理组织学变化等。结果：玉郎伞水提物对大鼠的一般状况、体质量增长、摄食量、血液学、血液生化指标、脏体系数、病理组织学变化无明显影响。结论：在本试验条件下，玉郎伞水提物在20 80g生药?kg-1?d-1属于安全剂量范围。 　　【关键词】 玉郎伞；水提物；大鼠；长期毒性 　　【中图分类号】R965 【文献标识码】A 【文章编号】2095-1752（2015）08-0020-04 　　Study on Chronic Toxicity of Water Extract from Yulangsan in rats 　　Shi Zhi,Chen Cunxia,Huang Renbin.School of Pharmacy， Guangxi Medical University，Nanning 530021 Guangxi，China 　　【Abstract】: Objective:To observe the adverse effects and extent of long-term gavage of Yulangsan on rats and to provide the reference for clinical use. Method: Half male and female 80 Wistar rats were randomly divided into three treatment groups ( Yulangsan water extract 20g/kg/day, 40g/kg/day, 80g/kg/day of raw drug ) and a normal control group and then intragastric administration was given once a day for 91 days continuously. The normal control group was treated with the same volume of normal saline. During the 14 days of administration and withdrawal recovery, observe the general conditions of each rat like sign and behavior, food intake including their secretions. At the end of the dosing period, 12 rats were randomly taken from each group (with half male and female), then the blood serum was taken. After that they were sacrificed and detect the hematology, blood biochemistry and measure the liver index. At the end of the recovery period, the remaining rats in each group will also sacrificed in the same matter. Result No obvious abnormalities was found on Yulangsan water extract low, medium and high groups (although there has statistically significance on serum level, blood biochemistry and liver index but still in the normal control range) Conclusion Under the experimental condit