采用卵子-胚胎移植技术分析研究家畜受精和早期发育研究.pdf

中国农业科技导报，2013，15(1)：65—70 JournalofAgriculturalScienceandTechnology Egg-EmbryoTransfer：AnAnalyticalToolfor VintageExperimentsinDomesticFarm Animals HunterR H F ．LIXi．he。， (1．InstituteforReproductiveMedicin，HannoverVetednaffUniversity，HannoverD一30559，Germany；2．ResearchCenterfor AnimalGeneticResourcesofMongoliaPlateau，InnerMongoliaUniversity，Hohhot010021，China；3．InnerMongoliaSaikexing ReproductiveBiotechnologyCo．Ltd．，MengniuDairyResearchandDevelopmentCenter，HohhotO11517，China) Abstract：In orderto clarify thefertilisation and early developmentorthe prorgession ofunfertilisedeggsin the oviductofdomesticfarmanimals，fourtransplantstudiesaredescribedinthestudy．① Pigeggstransplantedfrom ovulationsinducedduringthelutealphaseoftheoestrouscyclewerefertilisedintheoviductsofinseminatedoestrous animals．Bycontrast，pigeggsfrom oestrousdonorsbecamehighlypolyspermicwhentransplantedtotheoviductsof animalsforce—matedduringthelutealphase．② Pigembryosatthestageofhatchedbafstoeysts(7dand8d)could betransplantedsuccessfullytosynchronousrecipientsandfullembryonicdevelopmentbedemonstratedbetween19d and23 d．Thus，the exposed tropheetoderm ofenlargingembryoscould withstand the physicalmanipulation of recovery andtransplantation，andthelifespanofcorporaluteaintheunmatedrecipientscouldbeprolongedafter7d and8dtransfered．③ Bovineoocytesaspiratedfrom2～6mnldiameterGraafianfolliclesandmaturedinvitrowere fertilisednormally in the oviductsofinseminated recipientheifers，demonstratingthe potentialofslaughterhouse ovariesforthegenerationofembryos．④ Transplantingequineeggstoapigoviduct，inwhicheggdescenttothe uterusrequiresonly46—48h，didnotrevealaretardedprorgessofdegeneratingunfertilisedhorseeggs，suggesting theinvolvementofnon—physicalfactorsinequineembryoprogressiontotheuterus．Prostaglandinsofembryonicorigin arenow k