大豆猝死综合症病菌检测技术研讨.pdfVIP

植物病理学报 ACTA PHYTOPATHOLOGICASINICA 35(6)(ZK)：28-34(2005) 大豆猝死综合症病菌检测技术研究 吴品珊h，严进1，J．C．Rupe2，S．Giammaria2，E．J．Boza2 of AR (1中国检验检疫科学研究院动植物检疫研究所，北京100029；2University 72701 Arkansas，FayettevilleUSA) 摘要：大豆猝死综合症是美国等几个大豆主产国的一种较新的、危害性很大的大豆真菌病害，它分别由两种镰刀菌Fusari． um virgulifol,lne和Fusarium tucumaniae引致。本研究确立了从土壤中筛选、分离大豆猝死综合症病菌的选择性培养基 酶切，与mtDNA探针2u18和4u40杂交的RFLP图谱可成功的鉴定Fvirguliforme，F 此建立了，通过选择性培养基分离进口大豆中的土壤，进而以Southern杂交技术鉴定大豆猝死综合症病菌的检测方法。 关键词：大豆猝死综合症；检测 Thedetection for suddendeath of techniques syndrome WU pathogenssoybean Pin-shahl，YAN InstituteofAnimalandPlant Jinl，J．C．Rupe2，S．Giammaria2，E．J．Boza2(1 Quarantine．Chinese of and of AR AcademyInspectionQuarantine，AQSIQ，Beijing 72701 100029，China；2UniversityArkansas，FayettevilleUSA) Sudden Death causedFusarium Abstract：Soybean andFusariumtucumaniae Syndromeby virguliforme isa newand severe diseasedistributedinUnitedStatesandsome respectivelyrelatively soybean othermain countries．Amodifledselectiveculture soybeanproduce mediumnamedPCNBwasobtainedtoisolateand enumeratethe fromsoil．SomeChinese varietieswere sensitivetothe pathogens soybean proved pathogens． TotalDNAofthe2 andtheirsimilarones withEcoR withmtDNA pathogens digested I，werehybridized of2u18