结肠癌耐药细胞系sw480L-OHP的建立和生物学特性研究.docVIP

精品论文 参考文献 结肠癌耐药细胞系sw480L-OHP的建立和生物学特性研究 胡敏 　　（射洪县人民医院肿瘤科 四川 遂宁 629200） 　　【摘要】目的：建立结肠癌sw480耐奥沙利铂细胞系(sw480/L-OHP),并研究生物学特性。方法：应用人结肠癌细胞系sw480，以反复诱导、逐渐递增奥沙利铂(L-OHP)浓度的方法，体外培养建成sw480/L-OHP。用CCK-8法进行细胞增殖实验检测其多药耐药性；RT-PCR检测抗凋亡基因survive、多药耐药基因MDR1的表达水平；Western blot检测P-糖蛋白、MRP1的表达。结果:sw480/L-OHP对奥沙利铂耐药，并对多种抗癌药物产生交叉耐药性；与亲本细胞相比sw480/L-OHP的MDR1、survive的基因表达均显著升高；sw480/L-OHP的MRP1蛋白表达没有明显变化，但P糖蛋白表达显著升高。结论：sw480/L-OHP细胞对奥沙利铂耐药，并对多种化疗药物呈不同程度交叉耐药性特征，具备耐药细胞生物学特性，可用于对人结肠癌耐药机制与逆转等方面的研究。 　　【关键词】sw480；多药耐药；奥沙利铂；MDR1；MRP1 　　【中图分类号】R735.3 【文献标识码】A 【文章编号】2095-1752（2015）03-0016-03 　　Colon cancer drug resistance cell line sw480 / L - the establishment of OHP and biological characteristics research Hu Min. Shehong peoples hospital, Sichuan Suining 629200, China 　　【Abstract】Objective Establish sw480 colon cancer cell line oxaliplatin into resistance (sw480 / L - OHP), and study the biological characteristics. Methods Using human colon cancer cell line sw480, induced by repeated, gradually increasing oxaliplatin into L - (OHP) concentration, the method of in vitro culture built sw480 / L - OHP. Using method of CCK 8 cell proliferation experiment testing the multi-drug resistance; Rt-pcr detection of antiapoptotic gene survive, multi-resistant MDR1 gene expression level; Western blot test P - glycoprotein, the expression of MRP1. Results Sw480 / L - OHP of oxaliplatin into drug resistance, and produce cross resistance to a variety of anti-cancer drugs; Compared with parental cells sw480 / L - OHP MDR1 gene expression were significantly elevated, survive. Sw480 / L - OHP MRP1 protein expression was not significantly change, but significantly increased expression of P glycoprotein. Conclusions Sw480 / L - OHP cells of oxaliplatin into drug resistance, and for a variety of chemotherapeutic drugs in different degrees of cross resistance characteristics of drug-resistant cell biological characteristics, can be used to people and reversal of drug resistance in colon cancer.