血浆（1→3）-β-D-葡聚糖在深部真菌感染中的诊断意义.docVIP

精品论文 参考文献 血浆（1→3）-β-D-葡聚糖在深部真菌感染中的诊断意义 刘芳 　　(沈阳市第四人民医院呼吸内科 沈阳辽宁 110031) 　　【摘要】目的 探讨血浆（1rarr;3）-beta;-D-葡聚糖(BDG)对于深部真菌感染的诊断意义。方法 对临床可疑深部真菌感染的患者同时采取血标本及无热源污染血标本各一份，分别作细菌培养和血浆（1rarr;3）-beta;-D-葡聚糖定量测定。血浆（1rarr;3）-beta;-D-葡聚糖定量测定采用MB-80微生物快速动态检测系统进行，统计学分析比较两种方法的一致性。结果 深部真菌感染组BDG含量（101.32plusmn;52.02pg/ml）与细菌败血症组（11.23plusmn;10.07pg/ml）、无细菌生长组（8.65plusmn; 0.21pg/ml）比较有显著统计学差异（t=4.4、3.907,Plt;0.01、0.01）。以20.0pg/ml作为血浆（1rarr;3）-beta;-D-葡聚糖定量测定判断深部真菌感染的cutoff值，其准确率89.52﹪、敏感度90.36﹪、特异度87.52﹪。结论（1rarr;3）-beta;-D-葡聚糖的检测具有较高的特异性和敏感性，可作为诊断深部真菌感染的重要辅助检测指标。 　　【关键词】（1rarr;3）-beta;-D-葡聚糖 深部真菌感染 检测方法 　　【中图分类号】R446.5 【文献标识码】A 【文章编号】1672-5085（2013）50-0051-02 　　Diagnosis Significance of Plasma (1rarr;3)-beta;-D-glucan in Patients with Deep Fungal Infection 　　Liu Fang 　　(Respiratory medicine department,The fourth people hospital of shenyang , Liaoning ,110001,P.R.China) 　　【Abstract】 OBJECTIVE To study the diagnosis significance of blood plasma (1rarr;3)-beta;-D-glucan measurement in diagnosis of deep fungal infection. METHODS Concentration of blood plasma (1rarr;3)-beta;-D-glucan were measured by Microbiology Kinetic Rapid Reader MB-80 within patients suspected with deep fungal infection. At the same time to detect blood culture . RESULTS Mean blood plasma (1rarr;3)-beta;-D-glucan of people in deep mycosis group is 101.32plusmn;52.02pg/ml.The bacterium septicaemia grouprsquo;result is 11.23plusmn;10.07 pg/ml,The negative patient grouprsquo;result is 8.65plusmn; 0.21 pg/ml. T-test analysis indicates the mean plasma (1rarr;3)-beta;-D-glucan of three groups have notable difference（t=4.4、3.907,Plt;0.01、0.01）. Based on the content of 20pg/ml, blood plasma (1rarr;3)-beta;-D-glucan showed accurate rate ,sensitivity, specificity of 89.52﹪、90.36﹪、87.52﹪respectively. CONCULION Blood plasma (1rarr;3)-beta;-D-glucan showed high sensitivity, specificity and had been the important index in rapid, early diagnosis of systemic fungal infect