响应面法优选虎耳草抗前列腺癌活性部位提取工艺.PDFVIP

·1768 · 中草药 Chinese Traditional and Herbal Drugs 第 44 卷 第 13 期 2013 年 7 月 响应面法优选虎耳草抗前列腺癌活性部位提取工艺 周 欣，陈华国，黄志金，先 春，杨世林 贵州师范大学，贵州 贵阳 550001 摘 要：目的 优选虎耳草 Saxifraga stolonifera 抗前列腺癌活性部位的提取工艺。方法 以岩白菜素、槲皮素-5-O-β-D-吡 喃葡萄糖苷、槲皮素-3-O-L- 鼠李糖苷、槲皮素以及总黄酮的提取率为考察指标，采用单因素试验结合 Box-Behnken 中心组 合设计方法进行提取工艺优化研究。结果 虎耳草抗前列腺癌活性部位的最佳提取工艺条件为回流提取，乙醇体积分数 60.04%，料液比例 1 ∶13.79，提取时间 89.98 min，提取温度 80 ℃，在此条件下岩白菜素、槲皮素-5-O-β-D-吡喃葡萄糖苷、 槲皮素-3-O-L- 鼠李糖苷和槲皮素以及总黄酮的提取率分别为97.15%、98.13%、98.37%、97.82%、96.33% 。结论 采用响应 面法优化了虎耳草中抗前列腺癌活性部位的提取工艺，对虎耳草的综合利用以及产业化发展提供了科学依据。 关键词：虎耳草；前列腺癌；活性部位；响应面法；提取 中图分类号：R284.2 文献标志码：A 文章编号：0253 - 2670(2013)13 - 1768 - 06 DOI: 10.7501/j.issn.0253-2670.2013.13.013 Optimization of extracting technology for active fractions from Saxifraga stolonifera with anti-prostate cancer activity by response surface method ZHOU Xin, CHEN Hua-guo, HUANG Zhi-jin, XIAN Chun, YANG Shi-lin Guizhou Normal University, Guiyang 550001, China Abstract: Objective To optimize the extracting technology for active fractions from Saxifraga stolonifera with anti-prostate cancer activity. Methods Using the extraction rates of bergenin, quercetin-5-O-β-D-glucopyranoside, quercetin-3-O-L-rhamnopyranoside, quercetin, and flavonoids as indexes, the single factor experiment combined with Box-Behnken design was used to optimize the extracting technology. Results The optimal extraction conditions were as follows: ethanol volume fraction 60.04%, liquid-solid ratio of 1 ∶13.79 for 89.98 min, and at the temperature of 80 ℃. Under these conditions, the extraction rates of bergenin, quercetin- 5-O-β-D-glucopyranoside, quercetin-3-O-L-rhamnopyranoside, quercetin, and flavonoids were 97.15%, 98.13%, 98.37%, 97.82%, and 96.33%, respectively. Conclusion The response surface analysis could optimize the extracting technology of active fra