【精品论文】鱼腥草叶片总RNA提取方法研究.pdf

, Jour al ofA huiAgr.i Sci. 2010, 38( 29): 16144- 16146 姜丽 李岩 RNA 1, 2 2* 2 1 1 吴成就 ,伍贤进 , 魏麟 , 刘选明, 黄星群 ( 1. , 410082; 2. , , 418008) [目的]筛选出适合鱼腥草(H outtuyn ia corda ta)叶片RNA 提取的方法[方法]以鱼腥草叶片为 料, 比较了经典RNA 提取试剂 盒Trizol试剂盒ATrizol试剂盒B以及富含多糖类 料RNA提取试剂盒提取鱼腥草RNA 的效果[结果] Trizol试剂盒ATrizol试剂 盒B和经典RNA 提取试剂盒难以提取出高质量的 RNA, 而富含多糖类 料RNA 提取试剂盒提出的RNA质量高, 完整性好, 可以满足 进一步分子生物学研究的要求[结论]普通 RNA 提取试剂盒难以提取出鱼腥草叶片的RNA 鱼腥草; 总 RNA; 提取方法 S412 A 0517- 6611( 2010) 29- 16144- 03 ExtractionM ethods of Total RNA fromH outtuyn ia corda ta Leaves WU Chengjiu et al ( Bioe ergy a d BiomaterialResearch Ce ter, I stitute of Life Scie ce a d T ech ology, H u a U iversity, Cha gsha, H u a 410082) Abstract [ Objective] The research ami ed to scree suitable extractio method of total RNA from H ou ttuyn ia corda ta. [M ethod] Four ex tractio methods, ClassicT otal RNA Isolatio K it, Trizol kitA, Trizol k it B a d EasyW ay RNA Amylose Pla tM iNiK itw ere compared for extracti g total RNA from H outtuyn ia corda ta. [ Result] It was d ifficult to extract totalRNA from H outtuyn ia cord a ta leaf by Trizol kitA, Tr izol kit B a d ClassicT otal RNA Isolatio Kitm ethod. T otal RNA extracted by EasyW ay RNA Amylose Pla tM iN iK it had h igh quality a d could be use for the further research of molecular biology. [ Co clusio ] The ordi ary RNA isolatio Kit was hard to extract totalRNA from H outtuyn ia corda ta leaf. Ke words H outtuyn ia corda ta; TotalRNA; Extracti g methods RNA, Norther 1. 2 cDNA cDNA 1. 2. 1 1 RNA (RACE) PCR ( DDRTPCR) : 600 lRLL, 1. 5m l, 6 l [ 1] RNA RNA, , ! , RNA , cDNA 100mg, , 65 10 m i [2] cDNA , (H out ()∀ 0. 5 BufferRL, tuy nia cordata ), , 5 m i #14 000 r/m i 5 m i, , RNA, RNA