小鼠论文：小鼠卵母细胞卵龄对孤雌激活质量的影响.docVIP

小鼠论文：小鼠卵母细胞卵龄对孤雌激活质量的影响 【中文摘要】探讨小鼠卵母细胞孤雌激活较为合适的卵龄,为小鼠孤雌激活的研究和发展、胚胎克隆及核移植重构胚激活提供比较规范的实验方法与数据。方法采用7%的乙醇与终浓度为5μg/ml细胞松弛素B联合激活的实验方法对CD-1?小鼠卵龄分别为14h、16h、18h、20h、22h的卵母细胞进行孤雌激活的研究;并对所形成的胚胎进一步的培养、形态学观察和分析。结果卵龄为18h组的卵裂率(95.42%)、囊胚形成率(48.43%)及囊胚孵出率(7.30%)分别高于卵龄为16h、20h组的卵裂率(86.40%、91.58%)及囊胚形成率(10.74%、13.13%);卵龄为16h、20h组的卵裂率及囊胚形成率分别高于卵龄为14h、22h组的卵裂率(83.37%、88.91%)及囊胚形成率(7.12%、9.60%);卵龄为16h与卵龄为20h组的卵裂率及囊胚形成率无显著差异,卵龄为14h与卵龄为22h组的卵裂率及囊胚形成率无显著差异。五组间比较,18h组较其他四组的卵裂率、囊胚形成率、囊胚孵出率方面均有显著差异(P0.05)。结论在上述几组卵龄的卵母细胞激活中:乙醇与细胞松弛素B联合对18h卵龄的小鼠卵母细胞进行孤雌激活5min,其卵裂率、囊胚形成率、囊胚孵出率等方面,与卵龄为14h、16h、20h、22h的小鼠卵母细胞的卵裂率、囊胚形成率、囊胚孵出率均有明显优势;(2)从一般形态观察,提示激活胚胎形态和发育均较正常,卵龄为18h激活的胚胎可能更具有后续发育潜能,这是激活重要所在;(3)此外,促排卵药物剂量、激活方式、培养体系、人员操作熟练程度等均是孤雌激活及后续发育影响因素。 【英文摘要】s This paper aims at finding a better oocytes age for mouse parthenogenetic activation, thus providing a more normalized experimental method and data for the Mouse Oocytes Parthenogenetic research, Embryo Cloning and the activation of the reconstructed nuclear transfer.Methods The experimental method of this research of combining 7% of ethanol to the Cytochalasin B final concentration is of 5 % , and then adopt the mixture to study the CD-1? oocytes age of mouse for 14h、16h、18h、20h and 22h of Oocytes Parthenogenetic activation respectively; before conducting further morphologic observation and analysis on formed embryo .Results parthenogenetic activation rate (95.42%)、blastula formation rate(48.43%), hatching rate(7.30%) of oocytes age of 18hs were higher than parthenogenetic activation rate (86.40%、91.58%)、blastula formation rate(10.74%、13.13%) of oocytes age of 16hs and 20hs;16hs and 20hs of oocytes age parthenogenetic activation rate、blastula formation rate were higher than 14hs and 22hs of oocytes age parthenogenetic activation rate(83.37%、88.91%)、blastula formation rate(7.12%、9.60%); There is no significant difference between oocytes age of 16hs and 20hs group oocytes age activation rate and blastocyst formation rate, also,there is no significant differe