医学论文-电针对力竭运动大鼠骨骼肌α-肌动蛋白基因表达的影响.docVIP

医学论文-电针对力竭运动大鼠骨骼肌α-肌动蛋白基因表达的影响 【摘要】? 目的 研究电针刺激对一次性力竭运动大鼠骨骼肌α-肌动蛋白基因表达的影响，初步探讨电针刺激提高运动能力、延缓运动性疲劳发生的分子生物学机理。方法 40只SD雄性大鼠分为4组（对照组、模型组、力竭组、电针组），对照组大鼠常规饲养3周后处死取材；其他三组进行3周的游泳训练，模型组大鼠在游泳训练后处死取材；力竭组大鼠在游泳训练后进行一次性力竭游泳后处死取材；电针组大鼠在游泳训练后进行一次电针刺激，然后进行一次性力竭游泳，力竭游泳后处死取材。记录力竭游泳时间，用RT-PCR方法测定骨骼肌α-肌动蛋白基因表达。结果 电针组的平均力竭游泳时间明显长于力竭组；模型组大鼠的骨骼肌α-肌动蛋白基因表达明显高于对照组和力竭组；电针组的α-肌动蛋白基因表达虽低于模型组，但高于力竭组和对照组。结论 （1）3周游泳训练可以提高大鼠骨骼肌α-肌动蛋白的基因表达；（2）电针刺激有助于促进力竭运动大鼠的骨骼肌α-肌动蛋白基因的表达，延长大鼠力竭游泳时间，提高大鼠的运动耐力，延缓运动性疲劳的发生。 【关键词】? 电针；肌动蛋白；力竭运动 ??? Effects of electroacupuncture on expression of α-actin mRNA induced by exhausted exercise in skeletal muscles of rats ??? 【Abstract】? Objective? To observe the effects of electroacupuncture on expression of α-actin mRNA induced by exhausted exercise in skeletal muscles of rats, and explore the molecular biological mechanisms of electroacupuncture in delaying the occurrence of exercise-induced fatigue.Methods? Forty male SD rats were randomly divided into four groups（control group; model group; exhausted exercise group; electroacupuncture group）. Control group was killed after raising for 3 weeks; other 3 groups were trained to swimming for 3 weeks, then model group was killed ; and electroacupuncture group were treated with electroacupunctrue before exhausted exercise; while exhausted exercise group attended exhausted exercise directly. Then the exhausted time was noted, and the expression of α-actin was tested by RT-PCR.Results? The mean time of exhausted swimming in electroacupuncture group was longer than that in exhausted exercise group. The skeletal muscle expression of α-actin mRNA in model group was obviously higher than that in control group and in exhausted exercise group; although the expression in electroacupuncture group was lower than that in model group, it’s higher than that in exhausted exercise group and in control group.Conclusion? （1）The expression of α-actin mRNA in skeletal muscle could be increased after 3 weeks’ swimming train；（2）compared with exhauste