医学论文-人参总皂苷和小檗碱对肺癌PG细胞分泌免疫抑制性细胞因子的影响.docVIP

医学论文-人参总皂苷和小檗碱对肺癌PG细胞分泌免疫抑制性细胞因子的影响 ??????????????????????? 作者：郝钰, 王萍, 吴珺, 邱全瑛 【摘要】? 目的：观察人参总皂苷（ginsenoside, Gs）和小檗碱（berberine, Ber）对肿瘤细胞分泌免疫抑制性细胞因子转化生长因子β1（transforming growth factorbeta1，TGFβ1）和前列腺素E2（prostaglandin E2，PGE2）的影响，探讨Gs和Ber对肿瘤免疫逃逸的作用机制。方法：首先建立人肺癌PG细胞与人T淋巴细胞株Jurkat细胞的共培养体系，随机分为Gs（100 μg/ml）组、Ber（10 μg/ml）组、空白对照组和Jurkat组。用Gs和Ber作用PG细胞24 h后与Jurkat细胞共培养，24 h后倒置显微镜下观察Jurkat细胞生长的状态；台盼蓝拒染实验计数共培养6 h和24 h后Jurkat活细胞数；流式细胞仪检测共培养24 h后Jurkat细胞的凋亡率；100、50、25 μg/ml的Gs和10、5、2.5 μg/ml的Ber分别处理PG细胞，并设空白对照组（PG细胞未经药物处理），酶联免疫吸附测定法和放射免疫法检测PG细胞上清液TGFβ1和PGE2的含量。结果：Jurkat细胞与Gs和Ber处理过的PG细胞共培养后，其数目较空白对照组明显减少，而凋亡率较空白对照组明显增加；Gs和Ber可以增加PG细胞TGFβ1的分泌，对PGE2的分泌无影响。结论：Gs和Ber可以增强PG细胞导致的Jurkat细胞生长抑制和凋亡，其机制可能与Gs和Ber增加PG细胞分泌TGFβ1有关。 【关键词】? 人参皂苷类; 小檗碱; 抑制因子, 免疫; 转化生长因子β1; 前列腺素E类 　　Objective: To observe the effects of ginsenoside (Gs) and berberine (Ber), two kinds of active components of traditional Chinese herbal medicine, on transforming growth factorbeta1 (TGFβ1) and prostaglandin E2 (PGE2) in PG cells. 　　Methods: Coculture system of human lung carcinoma cell line PG and human T lymphocyte cell line Jurkat was established. PG cells were treated with Gs (100 μg/ml) and Ber (10 μg/ml) for twentyfour hours, and then cocultured with Jurkat cells. After 24hour coculture, the state of Jurkat cells was observed with inverted microscope. The viable count of Jurkat cells was detected by trypan blue staining after 6 and 24hour coculture, and the apoptosis of Jurkat cells was evaluated by flow cytometry. PG cells were treated with 100, 50, 25 μg/ml Gs and 10, 5, 2.5 μg/ml Ber respectively, and the content of TGFβ1 and PGE2 in PG cells was detected by enzymelinked immunosorbent assay (ELISA) and radioimmunoassay (RIA) method. 　　Results: After coculture with PG cells treated with Gs and Ber, the number of Jurkat cells was less than blank control group, and the apoptosis rates of Jurkat cells in Gs and Bertreated groups were higher than blank control group. Gs and Ber could promote the