Salt mediated interactions between vesicles of the thylakoid lipid digalactosyldiacylglycerol英文文献.pdfVIP

Biochimica et Biophysica Acta 938 (1988)323-333 323 Elsevier BBA 73885 Salt-mediated interactions between vesicles of the thylakoid lipid digalactosyldiacylglycerol M u r r a y S. W e b b a, C.P.S. Tilcock b a nd B.R, G r e e n a Department of Botany and b Department of Biochemistry, University of British Columbia, Vancouver(Canada) (ReceivedI September1987) Key words: Digalactosyldiacylglycerol;Salteffect;Thylakoidstacking;Vesicleaggregation; Freeze-fractureelectronmicroscopy;Quasi-elasticfight scattering Interbilayer interactions between large unilameilar vesicles of the plant thylakoid galactolipid digalactosyi- diacylglycerol in aqueous salt solutions have been examined by light scattering, freeze-fracture electron microscopy, and X-ray diffraction. When suspended in aqueous salt solutions, vesicles of 100 nm diameter were found to aggregate in a rapid and reversible manner to yield aggregates greater than 1000 nm in diameter. Freeze-fracture electron microscopy showed these aggregates to consist of appressed, but not fused, vesicles. Quasi-elastic light scattering and turbidity experiments showed that aggregation was not due to charged impurities of the lipid behaving in accordance with electrostatic double double layer theory. Experiments testing the efficacies of various chloride salts indicated a strong correlation existed between ionic radius and ability of the salt to promote aggregation