Lentiviral Vectors Mediate Stable and Efficient Gene Delivery into Primary Murine Natural Killer Cells英文文献.pdfVIP

© The American Society of Gene Therapy original article Lentiviral Vectors Mediate Stable and Efficient Gene Delivery into Primary Murine Natural Killer Cells 1 1 Jimmy Tran and Sam KP Kung 1Department of Immunology, University of Manitoba, Winnipeg, Manitoba, Canada Natural killer (NK) cells are lymphocytes that provide an allow us to differentially study a protein’s function(s) in primary important line of defense against many types of micro- NK progenitors, resting and activated primary NK cells. The organisms, viruses and tumors. The development of an development of a simple and efficient methodology that sup- efficient gene transfer system for genetically modifying ports long-term and stable transduction of genetic materials primary murine NK cells will facilitate the studies of NK cell into primary NK cells and NK progenitors will allow a better differentiation, acquisition of self-tolerance, and induc- understanding of the mechanism of NK cell differentiation and tion of anti-tumor responses. In this study we used an receptor function(s) both in vitro and in vivo. In addition, the enhanced green fluorescent protein (EGFP)-expressing ability to manipulate NK cells directly to express novel receptors vector to carry out a systematic evaluation of the effi- or genetically engineered NK receptors will allow us to enhance ciency of lentiviral transduction of primary murine NK NK target recognition and redefine NK target specificities. cells with or without prior interleukin-2 (IL-2) activation. The major challenge in genetically