原创力文档- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
维普资讯
现代肿瘤医学 2008年 5月 第 l6卷第 5期 ·7l3·
rmhTRAIL诱导耐阿霉素细胞株 K562／A02凋亡及作用机制的实验研究
王雅茹 ，王福旭 ，温树鹏 ，杜行严 ，杨渤彦 ，张学军 ，杨世方
Themechanism oftheapoptosisoftheleukemiacelllineK562resistanttoAdriamycin
K562／A02inducedbyrmhTRAIL
WANGYa—ru，WANGFu—xu，WENShu—peng，DUXing—yan，YANGBo—yan，ZHANGXue—j．n，YANGShi
— · fang
DepartmentofHematology，TheThird日 豇 ，Baoding071000，China．
【Abstract】 Objective：Toinvestigatetheapoptosismechanismofmulti—drugresistantcelllineK562／A02(mdr
一 1)inducedbyrmhTRAIL．Methods：K562(mdr一1一)wfiscontrolled，morphologywgsobservedbeforenad
aftertreatmentofrmhTRALL；Flow c~ometerwlit8usedtoanaiy~ hteapoptoticpeakofvfriousconcenrtationsof 1Tn-
hTRAIL；MTrtoassayhtegrowthinhibitoryeffectofrmhTRAIL；ThedifferentmRNA expressionlevelsofDR4，
DR5，DcR1，DcR2ofK562na dK562／A02cellswere examinedbysemi—qunatitativereversetrna scriptionpolymer-
88echainreactionRT—PCR．Results：Th eapoptosisofK562，K562／A02cellsinducedbyrmhTRAILwlit8observed
atacytomorphologynagle，withtypicalcytmorphologlcaichnages；TheapoptosisratioofK562／A02groupsurpassed
K562(P0．05)；TheinhibitionofK562／A02cellssuprassedtheK562(P0．05)；K562／A02cellsexpressed
DR4mRNA na dDR5 mRNA higherhtna K562，hteexpressionofDcR1onK562／A02cellswas lowerhtna K562，
DcR2mRNA didnotexpressintwokindofcells．Conclusion：rmhTRAILCallinduceK562na dK562／A02cells印-
optosis；Th eefficacyofrmhTRAILtoinduceapo ptosisnadihnibitproliferationinK562／A02celllineismorepre-
dominnatthna hteirparentalcelllineinhtestudy；Th ehishexpressionofdeaht receptors nadhtelow expressionof
decoyreceptors couldbeoneofhtereasonshtatK562／A02cellswere more sensitivehtna K562．
【Keywords】tumornecrosisfacotr—relatedapoptosis—inducinglignad；mdr一1；K562；K562／A02；apoptosis；
r
您可能关注的文档
- Ridge Based Vessel Segmentation in Color Images of the Retina英文文献.pdf
- Bone morphogenetic proteins in the nervous system英文文献.pdf
- Chromatin Modifications by Methylation and Ubiquitination Implications in the Regulation of Gene Expression英文文献.pdf
- PRONEPHRIC LEUCOCYTES OF Cyprinus carpio ISOLATION, SEPARATION AND CHARACTERIZATION英文文献.pdf
- Filtration of aerosol particles by elliptical fibers a numerical study英文文献.pdf
- Inner Product Modules Over英文文献.pdf
- Liver specific microRNA 122 target sequences incorporated in AAV vectors efficiently inhibits transgene expression in the liver英文文献.pdf
- Cone beam computed tomography with a flat panel imager Effects of image lag英文文献.pdf
- Multispectral opto acoustic tomography of deep seated fluorescent proteins in vivo英文文献.pdf
- Concise Review Nanoparticles and Cellular Carriers Allies in Cancer Imaging and Cellular Gene Therapy英文文献.pdf
- COMMUNICATIONS 0.3 Second FLASH MRI of the Human Heart英文文献.pdf
- The fast multipole method for the wave equation a pedestrian prescription英文文献.pdf
- Lectin Reactivities as Intermediate Biomarkers in Premalignant Colorectal Epithelium英文文献.pdf
- pcDNA3 AFP TK Angio融合基因靶向性治疗人原发性肝癌的实验研究英文文献.pdf
- High Yield Growth of E.coZi at Different Temperatures in a Bench Scale Fermentor英文文献.pdf
- Evaluation of Ship Radiated Noise Level from Near Field Measurements英文文献.pdf
- A Simple and Effective Method to Analyze Membrane Proteins by SDS PAGE and MALDI Mass Spectrometry英文文献.pdf
- Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics英文文献.pdf
- Chipping Away the ‘Missing Heritability’ GIANT Steps Forward in the Molecular Elucidation of Obesity – but Still Lots to Go英文文献.pdf
- Seasonal dynamics of soil CO2 efflux in a conventional tilled wheat field of the Loess Plateau, China英文文献.pdf
文档评论（0）