High expression of a human lactoferrin in transgenic tobacco cell cultures英文文献.pdfVIP

Biotechnology Letters 25: 213–218, 2003. 213 © 2003 Kluwer Academic Publishers. Printed in the Netherlands. High expression of a human lactoferrin in transgenic tobacco cell cultures 1 1 2 2 3 Sun-Mee Choi , Ok-Sun Lee , Suk-Yoon Kwon , Sang-Soo Kwak , Dae-Yeul Yu Haeng- Soon Lee1,∗ Laboratories of 1Plant Cell Biotechnology, 2Environmental Biotechnology and 3Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Oun-Dong 52, Yusong, Daejeon 305-806, Korea ∗Author for correspondence (Fax: +82-42-860-4608; E-mail: hslee@kribb.re.kr) Received 6 November 2002; Accepted 22 November 2002 Key words: high expression promoter, human lactoferrin, recombinant protein, transgenic plant cell culture Abstract Transgenic Nicotiana tabacum cell lines were developed expressing the human lactoferrin gene driven by the oxidative stress-inducible peroxidase (SWPA2) promoter. Western blot analysis showed the accumulation of both the full-length human lactoferrin protein as well as a immuno-reactive truncated fragment. Accumulation of human lactoferrin as monitored by ELISA increased proportionally to cell growth and reached a maximal (up to 4.3% of total soluble proteins) at the stationary phase of growth. Protein extracts from transgenic tobacco cells exhibited antibacterial activity. Introduction plant cell suspension cultures is that recombinant pro- teins can be produced under certified conditions with Lactoferrin is an iron-binding glycoprotein with an ap- large scale-up at low cost, but yields are low compared proxima