几种低分子量有机酸对连作平邑甜茶幼苗光合与根系生长影响.doc

园艺学报，（）：– 2014411224892496 http: // www. ahs. ac. cn Acta Horticulturae Sinica E-mail: yuanyixuebao@126.com 收稿日期：2014–09–28；修回日期：2014–12–02 基金项目：国家现代农业产业技术体系建设专项资金项目（CARS-28）；山东省农业重大应用技术创新课题；教育部长江学者和创新团 队发展计划项目（IRT1155） 几种低分子量有机酸对连作平邑甜茶幼苗光合 与根系生长的影响 李俊芝，王功帅，胡艳丽，陈学森，毛志泉* （山东农业大学园艺科学与工程学院，作物生物学国家重点实验室，山东泰安 271018） 摘 要：以平邑甜茶（ Malus hupehensis Rehd . ）幼苗为试材，在盆栽条件下研究了田间实测浓度的有 机酸（草酸、酒石酸、柠檬酸和苹果酸）对连作幼苗光合特性、叶片保护性酶活性以及根系生长的影响。 结果表明，与对照相比，草酸可使平邑甜茶幼苗植株干质量和鲜质量分别增加 24.12%和35.72%。叶片净 光合速率、气孔导度分别提高32.27%和76.58%。并显著提高了过氧化物酶（POD）和过氧化氢酶（CAT） 活性。酒石酸则降低了平邑甜茶幼苗叶片的气孔导度40.22%，SOD、POD活性亦显著降低。试验结果还 表明，柠檬酸和苹果酸也促进了平邑甜茶幼苗的生长。综上，在本试验条件下，草酸能够减轻平邑甜茶 幼苗连作障碍现象，酒石酸有加重连作障碍趋势，柠檬酸和苹果酸对连作障碍不明显。 关键词：苹果；连作障碍；平邑甜茶；土壤有机酸；光合特性；保护性酶 中图分类号：S 661.1 文献标志码：A 文章编号：0513-353X（2014）12-2489-08 Effect of Organic Acids on Biomass and Physiological Characteristics of Malus hupehensis Seedlings Under Continuous Cropping LI Jun-zhi，WANG Gong-shuai，HU Yan-li，CHEN Xue-sen，and MAO Zhi-quan* （ College of Horticultural Science and Engineering ， Shandong Agricultural University ， State Key Laboratory of Crop Biology ， Tai’an ， Shandong 271018， China ） Abstract：This experiment was conducted to explore the effect of low molecular weight organic acids on the biomass and physiological characteristics of Malus Hupehensis Rehd. seedlings under continuous cropping. Seedlings were grown in pots were treated with oxalic acid，tartaric acid，citric acid，malic acid and the mixture of the these four organic acids. The effects of organic acids on the photosynthetic parameters，the activities of antioxidant enzymes and root system of Malus hupehensis Rehd. seedlings were investigated. Compared with controls，oxalic acid could significantly promote dry and fresh weight of plant by 24.12%，35.72%，respectively. The net photosynthesis rate，stomatal conductance were significantly increased by 32.27%，76.58%，re