毕业论文—SUR1基因组织定位.docx

摘要：芥子油苷是一类十字花科植物所特有的硫代产物，芥子油苷代谢与植物防御反应密切相关，且某些芥子油苷的降解产物具有很高的抗肿瘤活性，因而其代谢及调控机制的研究备受关注。随着生物信息学的发展以及芥子油苷合成途径中相关基因的鉴定，SUR1基因参与硫苷生物合成模式三个阶段中硫苷核心结构的形成阶段，SUR1表达的C-S lyase SUR1 能将 S-烷基硫代烷肟转换成硫代肟基酸。本研究以模式植物拟南芥为实验材料。采用USER fusion，构建载体，农杆菌转化，GUS染色等方式，观察其表达模式，完成其组织定位，结果用以和其他已知的芥子油苷合成路径中的一些相关的基因进行表达上的对比，通过这中方式探讨SUR1基因在芥子油苷的合成途径中的功能意义，以及SUR1基因时空表达的特异性。关键词：芥子油苷,拟南芥,SUR1 ,USER fusionAbstract: is a class of sulfur-containing secondary metabolism outcomes specialized in Cruciferous plants. Glucosinolates have an important role in plant defense and some of the glucosinolates degradation products can prevent cancer in humans. Today, the researching about the glucosinolates metabolism and its biosynthesis regulation is under the spotlight. Along the development of bioinformatics and the demonstration of the new genes which involve the biosynthesize of glucosinolates,，SUR1 take part in the process Constructing the glucosinolate core,which can happen non-enzymatically, the produced S-alkylthiohydroximatesare converted to thiohydroximates by theC-S lyase SUR1。In this study，we use the?model plant Arabidopsis?as experimental material,USER?fusionconstruct?vectors,?Agrobacterium-mediated transformation,GUS?,to observe?expression pattern and complete its?tissue localization.the results?are?usedand?some?other known?mustard oil?glycoside?synthesisroute?related?gene?expressioncontrast.the functional significance?of?the?SUR1?gene?in?the?biosynthesis?ofglucosinolates?this?way?SUR1?gene?temporal and spatial expression?specificity.Key wordGlucosinolates , Arabidopsis thaliana, SUR1 , USER fusion1前言1.1研究背景硫代葡萄糖苷即芥子油苷简称硫苷，是一种含氮、硫的化合物。芥子油苷广泛分布于16 个不同科的双子叶被子植物中, 它们分别是十字花科( Brassicaceae) 、藜木科( Bataceae) 、钟萼木科( Bretschneideraceae) 、白花菜科( Capparaceae) 、番木瓜科( Caricaceae) 、大戟科( Euphorbiaceae) 、环蕊科( Gyrostemonaceae) 、池花科( Limnanthaceae) 、辣木科(Moringaceae) 、瘤药树科( Pentadiplandraceae) 、商陆科( Phytolaccaceae) 、海桐花科( Pittosporaceae) 、木犀草科( Resedaceae) 、刺茉莉科( Salvadoraceae) 、烈味三叶草科( Tovariaceae)和旱金莲科( Tropaeolaceae)