rhepo改善慢性脑缺血大鼠认知功能的作用机制的分析word论文.docxVIP

RhEPO 改善慢性脑缺血大鼠认知功能的作用机制的研究 摘 要目的研究重组人促红细胞生成素（rhEPO）改善慢性脑缺血大鼠认知功能的作用 机制。方法将 18 只健康雄性 SD 大鼠经 Morris 水迷宫筛选合格后，结扎双侧颈总动脉（2VO）建立永久性慢性大鼠脑缺血模型，并将其随机分为模型组、rhEPO 组、 rhEPO +AG490 组，分别给予编号。于造模成功后第 3 天开始，将模型组、rhEPO 组、rhEPO+AG490 组在等时间点给予等剂量药物治疗，共给予 3 次药物干预。 8 周后行 Morris 水迷宫实验，观测 3 组大鼠的空间学习记忆能力,即定位航行实 验和空间探索实验。断头取脑，进行脑组织病理切片，HE 染色后观察皮层及海 马区的细胞形态学变化。结果（ 1 ） 行 为 学 测 试 结 果 显 示 rhEPO 组 平 均 逃 避 潜 伏 期 较 模 型 组 、 rhEPO+AG490 组明显改善，分别比较差异具有统计学意义(P0．05)，而模型组 与 rhEPO+AG490 组逃避潜伏期差异没有统计学意义(P0．05)，提示 rhEPO 能 改善慢性脑缺血大鼠学习能力，而其效果可以被 AG490 抑制。（2）HE 染色提示模型组、rhEPO+AG490 组大鼠皮层及海马区细胞形态学 改变较 rhEPO 组明显，说明 rhEPO 可以减少慢性脑缺血海马细胞凋亡，而其作 用可以被 AG490 抑制。结论1. rhEPO 可以改善慢性脑缺血大鼠的认知功能；2. rhEPO 可能通过激活 JAK/STAT 通路减少神经元的凋亡。关键词促红细胞生成素；AG490；鼻腔给药；定位航行实验；空间探索实验The Mechanism of Recombinant Human Erythropoietin Improve Cognitive Function in Chronic Cerebral Ischemia RatsAbstractObjectiveStudy the effects of recombinant human erythropoietin (rhEPO) ,by means of observing cognitive function in chronic cerebral ischemia rats.MethodsAfter screening qualified by Morris water maze , eighteen healthy male SD rats are ligated bilateral common carotid artery (2 vo) to build the chronic ischemia model. Then they are randomly divided into the model group, the rhEPO group and the rhEPO + AG490 group, who are randomly numbered in every group. Three days after being ligated bilateral common carotid artery, three groups of rats are weekly undergone the intranasal delivery or intraperitoneal injection at the same dose and at the same time, and are given three times of drug interventions in all. Eight weeks after being ligated bilateral common carotid artery, three groups of rats are observed spatial learning and memory ability by Morris water maze experiment, namely the navigation experiments and space exploration experiment. Then they are beheaded in brain and made brain tissue pathological slices by Hematoxylin-Eosin Staining, in order to observe cortex and hippocampus cell morphology.ResultsBehavior test results show: compared with