[药学]超高效液相色谱.pptVIP

高分辨串联质谱QTOF micro 灵敏度高(pg-fg级)和选择性强得到的质谱谱图数据完整、品质高。因而，在天然产物，新药开发，药代研究和蛋白质组学领域的定性、定量分析研究方面占有重要地位。 从HPLC-MS到UPLC-MS 灵敏度明显提高 4.2 超高效液相色谱的展望 UPLC可以更快的速度和更高的质量完成以往HPLC的工作，为用户节省宝贵的时间和日常溶剂消耗，从而获得最大的投资回报。 UPLC的高分离度可从容面对复杂组份（如蛋白质与代谢组学等生化领域、天然产物）分离的挑战。 UPLC的高灵敏度可检测更加痕量的目标化合物。 UPLC的快速分析大量样品，实现高通量。 Development and validation of UPLC for the determination of phenolic compounds and furanic derivatives in Brandy de Jerez HPLC: Waters (Milford, MA, USA) system consisting of a model 616 pump, a 510 pump, a model 717 autosampler, and a model 996 photo-diode array detector. Column: a LiChrospher RP-C18 column (Merck) 25064 mm/ID with particle size of 5μm. Chromatographic conditions: 1 mL/min flow rate; 50μL injection volume; and eluents: A (5% methanol, 2% acetic acid, 93% water) and B (90% methanol, 2% acetic acid, 8% water). The gradient was as follows: 0 min 100% A, 20 min 90% A (curve 7), 60 min 25% A. The column was re-equilibrated before the next injection. Prior to their injection in the HPLC, the samples were filtered through a membrane of 22μm pore size (Millipore). HPLC analysis HPLC: Waters Acquity UPLC system coupled with a photodiode array detection method. Column: An Acquity UPLC BEH C18 column (1006 2.1 mm/ID, with 1.7μm particle size), also from Waters, was used. The column temperature was maintained at 47℃. Chromatographic conditions: A (3%ACN, 2% acetic acid, 95% water) and B (85% ACN, 2% acetic acid, 13% water), with a flow rate of 0.7 mL/min, giving a maximum back pressure of 10 400 psi, which is within the capabilities of the UPLC. The injection volume was 2.5μL. The 6.5 min gradient was as follows: 0 min, 100% A, 3 min, 90% A (curve 6), 4 min, 90% A, 6.5 min, 25% A (curve 6). Finally, the column was washed with 100% B for 3 min and equilibrated with 100% A for 3 min. All the samples were filtered through 0.22μm nylon filters fromScharlab. UPLC analysis Identification of each compound: comparing retention times and UV-Vis spectra of the peaks in wine with