利用基于PCR的分子标记区分普通小麦-提莫菲维小麦渐渗系.pdfVIP

HEREDITAS (Beijing) 2007 10 , 29(10): 1256―1262 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-1256 PCR - 纪剑辉, 曹爱忠, 王海燕, 覃碧, 王苏玲, 孔芳, 陈佩度, 刘大钧, 王秀娥 , 210095 : 采用定位于小麦2B 染色体上的72 对分子标记对含小麦抗白粉病基因Pm6 的8 份普通小麦（T.aestivum L. ）-提莫菲维（T.timopheevii zhuk. ）渐渗系材料进行分析, 通过分子标记标图确定8 份材料中渗入的提莫菲维 小麦染色体片段的大小, 同时结合连锁图谱对这些材料进行了遗传和物理标图。参考本研究所用的分子标记在 染色体2B 上的定位结果, Pm6 基因被位于2B 染色体长臂近末端2BL-6 区域, 提莫菲维小麦2G 染色体渐渗片 断长度由短到长排列顺序为: IGV1-465IGV1-464IGV1-463IGV1-468(IGV1-448 、IGV1-458)IGV1-474 IGV1-466 。研究结果与陶文静(1999)采用RFLP 标记标图的结果基本吻合, 进一步确认IGV1-465 渐渗片段最 小。本研究所鉴定的基于PCR 的分子标记还可以用于区分该套渐渗系, 这为这套材料在育种中的分子标记辅助 选择和Pm6 基因的克隆奠定了基础。 : 小麦; 提莫菲维小麦; 白粉病; Pm6 ; 渐渗系 Discrimination of the Triticum aestivum-T. timopheevii introgression lines using PCR-based molecular markers JI Jian-Hui, CAO Ai-Zhong, WANG Hai-Yan, QIN Bi, WANG Su-Ling, KONG Fang, CHEN Pei-Du, LIU Da-Jun, WANG Xiu-E State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China Abstract: In order to determine the fragment size of Triticum timopheevii chromosome segments introduced into wheat background and physically map the Pm6 gene, a total of 72 primers (including SSR and STS primers) were used to analyze the eight introduced introgression lines containing Pm6 gene. Referring to the available mapping information of the ana- lyzed markers on chromosome 2B, Pm6 was physically located in distal part of the long arm of chromosome 2B at the re- gion of Bin 2BL-6. The introgressed fragment sizes of the chromosome 2G in different introgression lines was determined to be as follow (from short to long):