SNP标记用于猪肉产品DNA溯源.doc.DOCVIP

SNP标记用于猪肉产品DNA溯源 吴潇吕贝贝王金斌蒋讳李鹏武国干唐雪明 上海市农业科学院生物技术研究所 釆用基于个体基因组DNA序列的差异而进行个体识別的DNA溯源技术，建立DNA 溯源系统对肉产品的质量安全进行控制。在实验群体中（10个品种，233个个体） 检测了 33个新单核苷酸多态性（single nucleotide polymorphism, SNP）标记 的遗传多样性，通过杂合度计算筛选出6个SNP标记可用于猪肉产品DNA溯源。 进一步在屠宰场采样进行溯源模拟实验，结果表明筛选的18个SNP标记（6个 新SNP标记结合己有的12个SNP标记）能有效区分100头猪个体，随机抽取的 10个个体的组织样品都能通过基因型比对找到对应的个体。本研究可为早日建 立猪肉产品的DNA溯源系统提供一定的技术参考。 关键词： 猪肉产品溯源;DNA溯源技术;SNP标记;个体识别; R-mai1:qwuxiao@126. 基金：闵行区产学研合作计划项目（2016MH256） Development and Application of Pork Traceability System Based on SNP Markers WU Xiao Ux Beibei WANG Jinbin JIANG Wei LI Pen WU Guogan TANG Xueming Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences; Abstract： DNA traceability technology is based on DNA sequence differences between individuals for individual identification and the tracing of meat back to an individual animal. Establishing a DNA-based traceability system is important for the safety and quality of meat products. The genetic diversity of 33 new single nucleotide polymorphism (SNP) markers was detected in a test group (233 individuals，10 varieties) and 6 SNP markers, which are useful for pork product traceability, were obtained by heterozygosity calculation. A total of 18 SNP markers (6 new SNP markers in combination with 12 SNP markers) could effectively distinguish between 100 individuals collected from a local slaughter house in traceability simulation experiments. The corresponding individuals for tissue samples from 10 random individuals were found by genotype.This study has shown that SXP markers could be used for individual identification and pork traceability. Keyword： pork traceability; DNA traceability technology; SNP markers; individual identification; 吴潇，吕贝贝，王金斌，等.SNP标记用于猪肉产品DNA溯源[J].食品科学， 2017，38 (24) :278-282. D01:10. 7506/spkx1002-6630-201724045. . spkx. net . WU Xiao, mBeibei, WANG Jinbin, et al. Development and application of pork traceability system based on SNPmarkers[J]. Food Science, 2017, 38 (24) :278~282. (in Chinese with E