几种阴离子对嗜酸氧化亚铁硫杆菌生长和硫氧化活性的影响.doc

几种阴离子对嗜酸氧化亚铁硫杆菌生长和硫氧化活性的影响 张成桂，张倩，王晶，张瑞永，何环，夏金兰(，邱冠周 中南大学资源加工与生物工程学院，生物冶金教育部重点实验室，湖南，长沙，410083 摘要：以嗜酸硫氧化细菌中的模式菌株嗜酸氧化亚铁硫杆菌A. ferrooxidans为研究对象，报道几种阴离子（NO3-、SO42-、Cl-和PO43-）钾盐对A. ferrooxidans生长和硫氧化活性影响的一般性规律，结果表明：不同的阴离子对A. ferrooxidans硫氧化表观活性影响效应不同，其影响顺序由大到小依次为：Cl- NO3- SO42- PO43-；在高浓度PO43-胁迫(300 mmol/L)下细菌表达蛋白相对与正常生长情况下的细菌蛋白质的总数量相对减少，可能是正常生长的细菌中一些参与生化反应的蛋白质的表达在胁迫条件下收到抑制，但在胁迫条件下细菌能表达一些特异性蛋白来调整和适应对无机阴离子胁迫效应。 关键词：嗜酸氧化亚铁硫杆菌；氯离子；硝酸根离子；硫酸根离子；磷酸盐胁迫 中图分类号: TF18 文献标识码：A 文章编号：Effect of Some Anions on Oxidation of Elemental Sulfur by Acidithiobacillus ferrooxidans ZHANG Cheng-Gui, ZHANG Qian, WANG Jing, ZHANG Rui-Yong, HE Huan, XIA Jin-Lan(, QIU Guan-Zhou School of Minerals Processing and Bioengineering, Central South University, Key Laboratory of Biometallurgy of Ministry of Education of China, Changsha 410083, China Abstract: The oxidation of elemental sulfur by Acidithiobacillus ferrooxidans ATCC 23270 was studied in the presence of different concentrations of some typical inorganic anions including sulfate, phosphate, chloride, and nitrate. The results showed that the growth and sulfur oxidation activity were obviously impacted by these anions, and the inhibition effect sequence as Cl- NO3- SO42- PO43-. There were exit differential expressed proteins spot in the 2-DE electrophoresis of total protein of A. ferrooxidans between PO43- threatening condition (300 mmol/L) and normal condition. These proteins may be take part in the biochemical reaction at normal condition, or response to various anions and adapt to the environment threatening condition respectively. Keywords: Acidithiobacillus ferrooxidans; chloride; nitrate; sulfate; phosphates threatening 硫化矿分解时产生大量的元素硫和其它含硫化合物，它们经嗜酸硫氧化细菌（包括A. ferrooxidans）氧化为终产物硫酸。这个过程十分复杂，受各种因素，包括物理、化学和生物因素的影响。A. ferrooxidans以氧化亚铁离子、元素硫或含还原态硫化合物获得能量而生长嗜酸硫氧化细菌之一，是重要的生物浸出功能菌 [1-2] 。A. ferrooxidans生存在极端酸性环境下，属自养革兰氏阴性细菌，最适生长温度为30℃左右，其中在一定物理化学条件下，如：在一定的温度、大气压、营养离子和实验菌种的条件下，则生物硫氧化过程受环境中各种阳离子和阴离子的影响 [3-5] 。已有的研究表明，A. ferro