毛细管电泳文献报告.pptVIP

文献报告 Separation and determination of four ganoderic acids from dried fermentation mycelia powder of Ganoderma lucidum by capillary zone electrophoresis Journal of Pharmaceutical and Biomedical Analysis Na Ding, Qing Yanga Sha-Sheng Huang, Liu-Yin Fan, Wei Zhang, Jian-Jiang Zhongc,Cheng-Xi Cao Laboratory of Analytical Biochemistry and Bioseparation, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China Abstract Ganoderic acids (GAs) were bioactive secondary metabolites produced by a traditional mushroom Ganoderma lucidum. We describe a simple and efficient method for the separation and quantitative determination of four GAs. Under the optimum conditions, the four GAs reached the baseline separation in 9 min with Glycyrrhetinic acid (GTA) as internal standard. Chemical structures of GA-T, GA-Mk, GA-Me, GA-S Experimental Optimization of conditions Analysis of real samples Validation Optimization of conditions Selection of running buffer and buffer pH value Effect of organic additive Effect of buffer concentration and applied voltage Optimized separation conditions 25mM pH 9.0 sodium borate buffer with 57% ACN (v/v), 27.5 kV applied voltage, 55cm total length (46cm to the detector) and 75um i.d. capillary, 245nm detection wavelength, 10 s 13mbr pressure injection. Analysis of real samples Calibration curves : 0.5, 1.0, 5.0, 10.0, 20.0, 30.0, 50.0, 60.0, 80.0 and 100.0L of the four GAs solutions (1.0 mg/mL) and 20.0uL internal standard solution(1.0 mg/mL)were added into blank sample powder in sequence from low to high concentration and extracted. Internal standard Blank sample powder the blank sample was the matrix of G. lucidum mycelia powder but free of GAs, it was obtained by collecting the G. lucidum mycelia powder which had been extracted the four ganoderic acids, and no GAs peak appe