[基础医学]分子生物学RNA干扰RNAi.pdf

RNAi的机制及其应用 (Mechanism and applications of RNA interference) Contents 一. RNAi的发现 二. RNAi 的作用机制 三. RNAi的特点 四. RNAi的应用领域 五.如何用siRNA沉默靶基因表达 六. siRNA应用实例 一.RNAi的发现 ( The discovery of RNA-mediated interference ) In 1990: Dr. Jorgensen 共抑制现象(cosuppression) Richard Jorgensen, PhD. Univ. of Arizona RNAi Innovator Awardee an interesting discovery cosuppression 强启动子--色素基因 petunias Wild type The petunias contain transgenes that induce suppression of both transgene and endogenous gene expression, giving rise to the unpigmented white areas of the flower. Kenneth Kemphues Professor of Genetics Su Guo Cornell graduate student Four-cell for distribution of Two-cell to visualize Two-cell mitotic spindles germline-specific granules. wild-type embryos par-1 mutant embryos Su Guo was working on identifying the gene called par-1 that is disrupted in a C. elegant worm mutant. In worms with the mutated par-1 gene, the embryonic cells fail to undergo normal, non-symmetrical division. Antisense RNA a crazy experiment sense or antisense RNA Most people thought RNA of PAR-1 gene would be easily degraded in an animal and have no effect. injection Guo Su,Kemphues K J. Par