海南淮山茎腐病病原生物学特性研究-广东农业科学.PDF

广东农业科学 2014 年第 9 期 95 海南淮山茎腐病病原生物学特性研究 赵志祥 陈 圆 肖 敏 肖彤斌 陈绵才 海南省农科院农业环境与植物保护研究所/海南省植物病虫害防控重点实验室袁海南海口 571100冤 要 淮山茎腐病是近年来发生在海南淮山中的一种重要病害袁对海南海口尧临高等地的淮山生产造成严重 危害遥 通过菌丝生长速率法测定了培养基尧温度尧酸碱度尧光照对淮山茎腐病菌菌丝生长的影响袁同时袁测定了湿度和 养分对孢子萌发的影响遥 结果表明院PDA 培养基最适合菌丝的生长曰菌丝能够生长的温度范围为 10~35益袁最适温度 为 25益袁致死条件为 50益处理 10 min 或 55益处理 5 min 曰最适 pH 值为 7.0 袁菌丝能够生长的pH 值范围为 5~10 曰光照 条件则以黑暗条件下最佳袁而孢子萌发的最佳相对湿度为 100% 袁溶液成分对孢子萌发的影响不大遥 关键词 淮山茎腐病菌曰 菌丝生长曰 孢子萌发 中图分类号 Q93-33 文献标识码 A 文章编号 1004-874X 2014冤09-0095-04 Study on biological characteristics of dioscorea stem rot disease in Hainan ZHAO Zhi-xiang 袁 CHEN Yuan 袁 XIAO Min袁 XIAO Tong-bin袁 CHEN Mian-cai Ins titute of A gro-Env ironme nt Plant Protec tion, Hainan A c ade my of A gric ultural Sc ie nc es/ Hainan Key Laboratory for Control of Plant Diseases and Inse c t Pe s ts , Haikou 571100 袁 China冤 Abstract: Dioscorea stem rot disease is a serious disease infected dioscorea in Hainan recently . At present, it has been broken out in Haikou and Lingao of Hainan, and caused serious economic losses to the dioscorea production in Hainan. In order to understand its biological characteristics, and to prov ide references for prevention and control of this disease, the method of mycelium growth rate was used to determine the influence of medium, temperature, pH value and illumination on mycelium growth. A lso, the influence of moisture and nutrient on spore germination was assayed . T he results showed that the most suitable culture medium was PDA and optimum temperature was 25益. T he