分子伴侣促进截短型人乳头瘤病毒58亚型l1蛋白在大肠杆菌中的可溶性表达.pdfVIP

Human PapillomavirusHPVDNA HPV 58 HPV 58 HPV GlaxosmithklineGSKHPV HPV16/18 HPV58 HPV 58 L1 HPV 58 Escherichia coli E.coliBL21 pTf16 E.coli BL21 pGEX-6P-HPV58-L1 GST-HPV58 L1 pTf16 IPTG L- L- 2.0 mg/ml L- 37 220 rpm 20 min 1.0 mmol/L IPTG18 24 hGST Prescission Protease GST SDS Western blot 50 KD HPV 58 L1 90%HPV 58 L1 ELISA 40 μg/20 μg/ PBS 1:64001:3200 HPV 58 HPV 58 L1 pTf16 Abstract Human papilloma virus is a kind of double stranded DNA virus, which has many subtypes. According to the difference of the infection, it can be divided into low risk type and high risk type. It causes common warts, flat warts, genital cancer, prostate cancer, oral cancer, cervical cancer, and other diseases through the skin and mucous membranes of the human body. In this paper, the study of the HPV 58 subtype is high-risk, and cervical cancer and cervical intraepithelial neoplasia is closely related to the occurrence of the infection of the HPV 58 subtype. Among women with cervical lesions in Asia, the rate of HPV 58 infection is high. And it is a serious threat to the health of the majority of women. However, domestic HPV vaccine research is relatively backward, there is no mature vaccine products, only the GSK two HPV (HPV16/18) vaccine in China. China is a high incidence of cervical cancer, the urgent need for independent research and developmen