(精选)miRNA教学课件.pptVIP

miR-34 genes are upregulated in the presence of p53 Validation of p53 dependent miR-34 induction Increasing p53 expression Ionizing radiation induces miR-34 family in a p53 dependent manner Overexpression of miR-34 family blocks proliferation and induces cellular senescence in IMR90 fibroblasts Cell cycle regulators are overrepresented among miR-34 targets miRNAs are dysregulated in cancer miRNAs are repressed as a class in tumors. However, a few specific clusters are activated Other canonical cancer pathways probably have miRNA components that have yet to be identified Since miRNAs are important as a class in cell differentiation and proliferation, in the future they may be used as diagnostic markers and therapeutic agents for cancer References 1. L He and Hannon GJ. “MicroRNAs: small RNAs with a big role in gene regulation” Nature Reviews Genetics 5 (2004): 522-31 2. RS Pillai et al. “Repression of protein synthesis by miRNAs: how many mechanisms?” Trends Cell Biol. 2007 Mar; 17(3):118-26 3. DP Bartel “MicroRNAs: Genomics, Biogenesis, Mechanism, and Function” Cell 2004 Jan 23; 116:281-297 4. AA Aravin et al. “Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in D. melanogaster germline” Curr Biol 2001 Jul 10; 11(13):1017-27 5. Horvitz and Sulston. “Isolation and genetics characterization of cell lineage mutants in the nematode Caenorhabditis elegans” Genetics 1980 Oct; 96(2):435-54 6. RC Lee et al. “The C elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14” Cell 1993 Dec 3; 15(5):843-54 7. VN Kim and Nam JW. Genomics of microRNAs” Trends in Genetics 200 Mar; 22(3):165-73 8. K Chen and Rajewsky N. “The evolution of gene regulation by transcription factors and microRNAs” Nature Reviews 8 (2007):93-103 9. AJ Levine et al. “The p53 pathway: What questions remain to be explored?” Cell Death Differ 2006 13:1027-36 10. KB Spurgers et al. “Identification of cell cycle regulatory genes