(精选)RNAi and miRNA教学课件.pptVIP

Both at Cornell Kemphues: Professor of Developmental Biology at Cornell; continued in Par proteins; ~44 par related papers published in last 23 years; involved in all known par proteins Guo: UCSF Neuroscience faculty Studying embryonic development when reported interesting results that added to the RNAi background Came to be cited as an example of RNAi before the term was coined three years later by Fire and Mello microRNAs: Biogenesis and Function small (21-25nt) non-coding RNAs with regulatory function derived from precursor transcripts with extended stem-loop structures Drosha Nucleus Pre - microRNA Pri - Dicer Cytoplasm Pre - microRNA microRNAs: Biogenesis and Function small (21-25nt) non-coding RNAs with regulatory function derived from precursor transcripts with extended stem-loop structures Dicer Ago2 RISC VIG TSN dFMR1 Cytoplasm microRNA microRNAs: Biogenesis and Function small (21-25nt) non-coding RNAs with regulatory function derived from precursor transcripts with extended stem-loop structures Dicer Cytoplasm Ago2 RISC VIG TSN dFMR1 AAAAAAAAAA CAP mRNA target microRNAs: Biogenesis and Function small (21-25nt) non-coding RNAs with regulatory function derived from precursor transcripts with extended stem-loop structures Dicer Cytoplasm Ago2 RISC cleavage AAAAAAAAAA CAP VIG TSN dFMR1 mRNA target microRNAs: Biogenesis and Function small (21-25nt) non-coding RNAs with regulatory function derived from precursor transcripts with extended stem-loop structures Dicer Cytoplasm Ago2 RISC VIG TSN dFMR1 AAAAAAAAAA CAP mRNA target AAAAAAAAAA CAP translational inhibition small (21-25nt) non-coding RNAs with regulatory function derived from precursor transcripts with extended stem-loop structures microRNAs: Biogenesis and Function Nucleus Ago1 RITS Tas3 ? CHP1 DNA target ? CH3 CH3 CH3 CH3 CH3 CH3 microRNAs: Biogenesis and Function small (21-25nt) non-coding RNAs with regulatory function derived from precursor transcripts with extended stem-loop struct