液相色谱_质谱分析葡萄籽提取物中原花青素_苏叶萍.pdfVIP

2010 11 29 11 # 1479# # 药品质量控制# - 1 2 2 1 2 苏叶萍 , 杜 平 , 金建秀 , 许巧霞 , 刘洪海 ( 1. , 832002; 2. , 830088) [ ] 采用快速高分离度液相色谱-串联质谱( RRLC-M SM S) 法分析 萄籽提取物中原花青素的化学成 分 萄籽提取液经HPD 400A 大孔吸附树脂纯化后, 采用 RRLC-M SM S 法分离, 并根据质谱信息分析其化学成 分 分离检测出 萄籽提取物中原花青素的 3 种单体, 初步分离鉴定出 9 种二聚体5 种三聚体和 1种四聚体 采用 RRLC-M SM S 法可以较好地分离分析 萄籽提取物中原花青素的主要成分 [] 原花青素; 萄籽; 质谱; 色谱法, 高效液相 [ ] R2 86; R 927. 1 [ ] A [] 1004 - 07 81( 20 10) 11- 1479- 03 Analysis ofProanthocyanidins inGrape Seeds by RRLC-MSMS 1 2 2 1 2 SU Y e-p ng , DU P ng , JIN J an-x u , XU Q ao-x a , L IU H ong-ha ( 1. Colleg e of Pharmacy, Sh ihez i University, Sh ihez i 832002, Ch ina; 2. Institute of X inj iang ChalkisN atural P lant, Urum q i 830088, Ch ina ) ABSTRACT Objective T o assay the chem cal compos t ons of proanthocyan d ns n grape seeds by RRLC-M SM S. M ethods The grape seeds extract on w as pu r f ed by H PD 400A m acroporou s adsorpt on res n, separa ted by RRLC-M SM S and chem cal com pos t ons o fw h ch w ere ana ly zed by nform at on of M SM S. Results T hree m onome rs, n ne d mm ers, f ve tr m ers and one tetram er of proantho cyan d ns n grape seeds w ere pre lm nar ly so lated and dent f ed. Conclusion The m ajor com pos t ons of proanthocyan d ns n g rape seeds cou ld be w ell sepa rated and ana lyzed by RRLC-M SM S. EYWORDS P roanthocyan d ns; G rape seed; M SM S; H PLC ( proanthocyan d ns) , , , [ 1] -( RRLC-M SM S) , ,