利用GUS基因表达观察启动子功能b教材课程.pptVIP

利用GUS基因表达观察启动子功能;二、实验原理1. 启动子（Promoter）;;The promoter region in higher eukaryotes. The TATA box is located approximately 30 base pairs from the mRNA start site. Usually, two or more promoter-proximal elements are found 100 and 200 bp upstream of the mRNA start site. The CCAAT box and the GC-rich box are shown here. Other upstream elements include the sequences GCCACACCC and ATGCAAAT. The core promoter, promoter-proximal elements, and distance-independent elements are all DNA sites that are recognized by sequence-specific DNA-binding proteins. The proper constellation of such trans-actin