土壤含水量对极小种群毛枝五针松幼苗抗氧化活性的影响.pdfVIP

热带亚热带植物学报 2016, 24(2): 182 ~ 188 Journal of Tropical and Subtropical Botany 土壤含水量对极小种群毛枝五针松幼苗抗氧化活 性的影响 * 康洪梅, 张珊珊, 杨文忠, 方波 (云南省林业科学院, 国家林业局云南珍稀濒特森林植物保护和繁育重点实验室，昆明 650201) 摘要：为探究水分对毛枝五针松(Pinus wangii)幼苗生理特性的影响，对不同土壤含水量下幼苗松针的抗氧化活性进行了研究。 结果表明，土壤含水量为田间持水量的40%~ 80%时，其POD 、SOD和CAT活性较强；随着处理时间的延长，细胞质膜渗透 性减弱，抗逆性变弱；脯氨酸、可溶性糖和蛋白质含量均以20%~ 40%的田间持水量较高；处理30 d后丙二醛含量随着土壤 含水量的增加而提高，在土壤含水量为田间持水量的40%时，丙二醛含量最低，抗逆性最强。因此，人工培育幼苗时，土壤 水分过多的时间尽量不要超过30 d ，控制土壤水分为田间最大持水量的30%~50% ，这样能提高幼苗存活率。 关键词：毛枝五针松；土壤含水量；幼苗；生理特性 doi: 10.11926/j.issn.1005-3395.2016.02.009 Effects of Soil Water Content on Antioxidant Activities of Pinus wangii Seedlings * KANG Hong-mei, ZHANG Shan-shan, YANG Wen-zhong , FANG Bo (Key Laboratory of Rare and Endangered Forest Plant of State Forestry Administration , Yunnan Academy of Forestry, Kunming 650201, China) Abstract: In order to explore the effects of soil water content on physiology characters of Pinus wangii seedlings, the antioxidant activities in pine needles were studied under different soil moisture. The results showed that the activities of POD, SOD, CAT were high under 40% -80% of field capacity. With prolong of time, the permeability of cell plasma membrane reduced, and stress resistance weakened. The contents of proline, soluble sugar and soluble protein were high under 20% -40% of field capacity. Malondialdehyde (MDA) content was gradually increased with the increment of soil water content for 30 d. When soil water content was 40% of field capacity, MDA content was the lowest and stress resistance of seedlings was the strongest. Therefore, the survival of seedlings could be improved under 30%-50% of field capacity, and the seedlings grown in too much soil water did not